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L6511

Lipopolysaccharides from Salmonella enterica serotype typhimurium

purified by phenol extraction

Synonym(s):

LPS

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About This Item

UNSPSC Code:
12352201
NACRES:
NA.25
MDL number:
MFCD00164404
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biological source

Salmonella enterica (Serotype typhimurium)

Quality Segment

200

form

lyophilized powder

purified by

phenol extraction

impurities

<3% Protein (Lowry)

color

white to faint yellow

solubility

water: 4.90-5.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

General description

This product is phenol extracted from Salmonella typhimurium. The source strain is ATCC 7823. This LPS has been used to induce NOS in rats and guinea pigs.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Lipopolysaccharides from Salmonella enterica serotype typhimurium purified by phenol extraction

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Sigma-Aldrich

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biological source

Salmonella enterica (Serotype typhimurium)

biological source

Salmonella enterica (Serotype typhimurium)

biological source

Salmonella enterica (Serotype typhimurium)

biological source

bacterial (Salmonella typhosa)

solubility

water: 4.90-5.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow

solubility

water: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

solubility

water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow

solubility

water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

impurities

<3% Protein (Lowry)

impurities

1-10% Protein (Lowry)

impurities

<1% Protein

impurities

<3% Protein (Lowry)

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pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How does heating the LPS at 95 degrees Celsius impact its activity? How long can LPS be incubated at 95?

    1 answer

    1. Heating lipopolysaccharides (LPS) at 95 degrees Celsius can lead to denaturation, which may reduce or eliminate biological activity. The duration for incubation at this temperature should be minimized to prevent significant loss of activity, typically not exceeding 30 minutes.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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