K3753

Krebs-Henseleit Buffer Modified

With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

• UNSPSC Code: 12352207

Properties

• Quality Segment: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• storage temp.: 2-8°C

Description

General description

Krebs-Henseleit Buffer Modified is a balanced salt solution (BSS) that is used in cell culture and plays a role in providing cells with water and inorganic salts for normal cell metabolism. In addition, it provides a buffering system to maintain the optimal pH of the medium and acts as an irrigating, transporting, and diluting agent to maintain the intra- and extra-cellular osmotic balance. BSS provides glucose as an energy source for cell metabolism.

Application

Krebs-Henseleit Buffer Modified has been used:

• to collect the rat brains for ex vivo brain slice experiments
• to measure calcium and sodium in rat pulmonary arterial endothelial cells (PAECs)
• to collect the uterine and forelimb blood vessel samples from horses

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.

Supplement with 2.1 g/L sodium bicarbonate.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable