Freeze/thaw studies of this material in solution have not been conducted. However, other sources indicate frozen aliquots should be limited to a maximum of one to two freeze–thaw cycles to maintain stability and potency. Stock solutions in ethanol or DMSO, stored at –20 °C and protected from light, are stable for several months. For short-term use up to 6 weeks, stock solutions in ethanol or DMSO to be stored at 2–8 °C protected from light. Please see the link below to review additional details available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/137/833/i0634dat.pdf
I0634
Ionomycin calcium salt
from Streptomyces conglobatus, ≥98% (HPLC), powder, Ca2+ ionophore
Synonym(s):
Ionomycin (calcium), SQ23377 calcium
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About This Item
Empirical Formula (Hill Notation):
C41H70CaO9
CAS Number:
Molecular Weight:
747.07
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.77
Beilstein/REAXYS Number:
1416163
MDL number:
Assay:
≥98% (HPLC)
Form:
powder
Storage condition:
desiccated, protect from light
Pricing and availability is not currently available.
Product Name
Ionomycin calcium salt from Streptomyces conglobatus, powder, ≥98% (HPLC)
Quality Segment
assay
≥98% (HPLC)
form
powder
storage condition
desiccated, protect from light
color
white to off-white
solubility
DMSO: 10 mg/mL, ethanol: soluble
antibiotic activity spectrum
Gram-positive bacteria
mode of action
cell membrane | interferes
storage temp.
2-8°C
SMILES string
C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)C\C=C\[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O
InChI
1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1
InChI key
WKRWUYKLUMMAKG-WYGBAUISSA-L
General description
Calcium Ionomycin is a polyether antibiotic made by Streptomyces conglobatus. It can remove Ca2+ and divalent cations from an aqueous into an organic phase. Ionomycin enhances the influx of Ca2+ by triggering store-regulated cation entry rather than acting directly on the plasma membrane. It can induce apoptosis, potentially by activating a hidden enzyme that responds to calcium. Since calcium salt of ionomycin serves as a highly efficient transporter of Ca2+, it offers substantial benefits for studying the transport of Ca2+ across biological membranes. Ionomycin is employed to balance intracellular and extracellular calcium ion concentrations for in situ calibrations of fluorescent indicators.
Additionally, it is a narrow spectrum antibiotic effective against Gram-positive bacteria, obtained in pure form from the fermentation broths of Streptomyces conglobatus. It is unique amongst known polyether antibiotics due to its exceptional UV absorption maximum at 300 nm and its high affinity for calcium ions.
Additionally, it is a narrow spectrum antibiotic effective against Gram-positive bacteria, obtained in pure form from the fermentation broths of Streptomyces conglobatus. It is unique amongst known polyether antibiotics due to its exceptional UV absorption maximum at 300 nm and its high affinity for calcium ions.
Application
Ionomycin calcium salt from Streptomyces conglobatus has been used as a media supplement to culture:
- peripheral blood mononuclear cells (PBMCs) for immunologic assessment[1]
- oocytes for parthenogenetic activation[2]
- T cell subsets for the detection of intracellular cytokines, carboxyfluorescein diacetate succinimidyl ester (CFSE) proliferation assay, and antigen-specific suppressive function assay[3]
- in combination with phorbol12-myristate 13-acetate (PMA) to stimulate T-cell activation in various cells [4]
- as sperm capacitation medium supplement to study its impact on in vitro fertilization and increasing the efficiency of bovine embryo development[5]
- as a positive control for the detection of intracellular Ca2+ in adipose-derived stem cells (ASCs)(4)[3]
Biochem/physiol Actions
Ca2+ ionophore that is more effective than A23187 as a mobile ion carrier for Ca2+.
Ca2+ ionophore that is more effective than A23187 as a mobile ion carrier for Ca2+; non-fluorescent; used to study Ca2+ transport across biological membranes; induces apoptotic degeneration of embryonic cortical neurons.
Ionomycin is an ionophore, widely used for validation and calibration of calcium analysis.
Preparation Note
Store ionomycin in powder form desiccated and protected from light at 2-8 °C. Under these conditions the product is stable for 3 years. Store stock solutions in ethanol or DMSO at -20 °C, protected from light. Under these conditions the stock solutions are stable for several months. For short term use (up to 6 weeks) the stock solutions maybe stored at 2-8 °C protected from light.
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This Item | |||
|---|---|---|---|
| assay ≥98% (HPLC) | assay ≥90% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) |
| form powder | form waxy solid | form powder | form powder |
| Quality Level 300 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. room temp | storage temp. −20°C |
| solubility DMSO: 10 mg/mL, ethanol: soluble | solubility DMSO: 10 mg/mL, methanol: 10 mg/mL | solubility DMSO: ≥10 mg/mL at warmed to 60 °C | solubility DMSO: 10 mg/mL, clear |
| storage condition desiccated, protect from light | storage condition OK to freeze, protect from light | storage condition desiccated | storage condition desiccated |
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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For how many freeze-thaw cycles are the ionomycin calcium salt dissolved in DMSO aliquots stable for use in cell culture/immunologic assessment?
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