H8280

N-Hydroxysuccinimidyl-Sepharose^™ 4 Fast Flow

matrix fast-flow highly cross-linked 4% beaded agarose

• Synonym(s): N-Hydroxysuccinimidyl-Agarose
• NACRES: NA.56
• UNSPSC Code: 23151817
• MDL number: MFCD00164950

Properties

• form: isopropanol suspension
• Quality Segment: 200
• extent of labeling: 16-23 μmol per mL
• technique(s): protein array: suitable
• matrix: fast-flow highly cross-linked 4% beaded agarose
• matrix active group: N-hydroxysuccinimide active ester
• matrix spacer: 6 atoms (when ligands are coupled by displacement of NHS)
• storage temp.: 2-8°C

Description

General description

N-Hydroxysuccinimidyl-Sepharose^™ 4 Fast Flow is a cross-linked and preactivated matrix obtained by mixing Sepharose 4 Fast Flow with 6-aminohexanoic acid through a spacer arm. Esterification with N-hydroxysuccinimide activates the terminal carboxyl group. Ligand-containing primary amino groups directly interact with this active ester to form a chemically very stable amide linkage.

Application

N-Hydroxysuccinimidyl-Sepharose^™ 4 Fast Flow has been used as a part of a magnetic particles-based visual detection system to achieve separation upon binding of the amplified gene. It has also been used to immobilize protein during pull-down assay.

Features and Benefits

• Specifically recommended for coupling of small amino-containing proteins and peptides in process-scale applications
• High level of pre-activation, which gives a high degree of substitution of the selected ligand
• NHS coupling method enables chemically stable ligand attachment

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• pictograms: GHS02,GHS07
• signalword: Danger
• hcodes: H225,H319,H336
• pcodes: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 3 - Flammable liquids
• wgk: WGK 1
• flash_point_f: 53.6 °F
• flash_point_c: 12 °C
• ppe: Eyeshields, Gloves, type ABEK (EN14387) respirator filter