This product is not tested for unit activity on a lot-to-lot basis. Historical data indicates a minimum activity of 1.7 x106 IU/mg. The material is tested for dose dependent stimulation of proliferation in CTLL-2 cells. The ED50 is reported in the Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/h7041#product-documentation
H7041
IL-2 human
≥98% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonym(s):
Interleukin-2, T-cell growth factor
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About This Item
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Form:
lyophilized powder
Mol wt:
monomer 15 kDa (glycosylated)
Pricing and availability is not currently available.
Product Name
Interleukin-2 human, IL-2, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested
biological source
human
Quality Segment
recombinant
expressed in HEK 293 cells
form
lyophilized powder
potency
≤5.0 ng/mL ED50
quality
endotoxin tested
mol wt
monomer 15 kDa (glycosylated)
packaging
pkg of 5X10 μg, pkg of 10 μg, pkg of 100 μg
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... IL2(3558)
General description
Interleukin-2 (IL-2) is an α-helical cytokine belonging to T-helper cell type 1. It has a molecular weight of 15kDa. The IL2 gene is mapped to human chromosome 4q27. IL-2 is originally called as T cell growth factor.
Application
Interleukin-2 human has been used to culture peripheral blood mononuclear cells (PBMC). It has been used to activate effector cell (PBMC) and to study antibody-dependent cell-mediated cytotoxicity.
Biochem/physiol Actions
Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lymphokine promotes long term growth of activated T cells and related cell types. IL-2 plays a role in the activation and proliferation of NK cells, induces γ-interferon and B cell growth factor secretion, and modulates the expression of the IL-2 receptor.
Interleukin-2 (IL-2) is essential for proliferation of natural killer (NK) cells, lymphocytes and macrophages. It plays a key role in the immune system functions such as NK cell-stimulated immune responses. IL-2 also mediates the formation and essential functions of regulatory T cells. It is implicated in autoimmune disease. IL-2 is associated with apoptosis and thus, possesses antitumor response. IL-2 mediates CD41+ T-cells differentiation into T-helper cell 1 and 2 effector subsets, preventing T-helper 17 differentiation.
Preparation Note
Human Interleukin-2 (IL-2) expressed in human HEK 293 cells is a glycosylated monomer with a molecular mass of 15 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line).
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This Item | |||
|---|---|---|---|
| recombinant expressed in HEK 293 cells | recombinant expressed in baculovirus infected Sf21 cells | recombinant expressed in E. coli | recombinant expressed in E. coli |
| biological source human | biological source human | biological source human | biological source human |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form solution |
| packaging pkg of 5X10 μg, pkg of 100 μg, pkg of 10 μg | packaging pkg of 5 μg | packaging pkg of 10 μg | packaging pkg of 200,000 U (2 μg, 1 ml) |
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Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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I would like to use this IL-2 for culturing cell lines. The protocol specifies adding 10 U/ml IL-2 (H7041), but the IL-2 available is in a 10 μg quantity. I need to determine the appropriate amount to add for culturing. Could you help me to calculate it?
1 answer-
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I want to know the activity (in unit) of this product. How can I converse EC50 to U/mg or U/mL?
1 answer-
This product is not tested against a WHO/NIBSC standard. The unit activity is not available. Please see the sample Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/H7041-10UG-PW0000173569.pdf
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