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EDEGLY

Enzymatic Protein Deglycosylation Kit

Synonym(s):

Glycosylation Release Kit

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
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Quality Segment

conjugate

(N-linked), (O-linked)

shipped in

wet ice

storage temp.

2-8°C

General description

Enzymatic protein deglycosylation kit contains all the enzymes and reagents required for the complete removal of N-linked and simple O-linked carbohydrates from glycoproteins. This kit is employed to cleave the complex Core 2 O-linked carbohydrates including those containing polylactosamine.

Application

Enzymatic protein deglycosylation kit has been used in:
  • protein deglycosylation[1]
  • deglycosylation of tissue factor pathway inhibitor (TFPI) α and β[2]
  • deglycosylation of native exo-β-(1,3)-glucanase EXG1[3]

Enzymatic Protein Deglycosylation Kit has been used for the deglycosylation of various enzymes.

Features and Benefits

Enzymatic protein deglycosylation kit has the following features:
  • two or more mg of glycoprotein is deglycosylated
  • single reaction at neutral pH
  • native & denaturing procedures
  • no degradation of protein
  • O-linked sugars containing polysialic acid are eliminated
  • control glycoprotein provided


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This Item
PP0510CS0008NDEGLY
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−70°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

-

shipped in

dry ice

shipped in

wet ice

conjugate

(N-linked)

conjugate

-

conjugate

-

conjugate

(N-linked)


Kit Components Only

Product No.
Description

  • PNGase F 1 mL/vial

  • O-Glycosidase 20 μL

  • α-2(3,6,8,9)-Neuraminidase 20 μL

  • Fetuin Control .5 mg

  • 5× Reaction Buffer .2 mL

  • Denaturation Solution .1 mL

  • Triton X-100 .1 mL

See All (9)

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3



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