D8783
7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt
10 mM in H2O
Synonym(s):
−N7-dGTP, 7-Deaza-dGTP
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About This Item
Empirical Formula (Hill Notation):
C11H17N4O13P3
CAS Number:
Molecular Weight:
506.19
NACRES:
NA.52
PubChem Substance ID:
UNSPSC Code:
41106305
MDL number:
Assay:
≥95% (HPLC)
Biological source:
Porcine muscle, rabbit muscle
Form:
liquid
Storage temp.:
−20°C
Quality Level
biological source
Porcine muscle, rabbit muscle
assay
≥95% (HPLC)
form
liquid
feature
PCR Additives
concentration
10 mM in H2O
technique(s)
PCR: suitable
color
colorless
shipped in
dry ice
storage temp.
−20°C
SMILES string
NC1=NC(=O)c2ccn(C3CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O3)c2N1
InChI
1S/C11H17N4O13P3/c12-11-13-9-5(10(17)14-11)1-2-15(9)8-3-6(16)7(26-8)4-25-30(21,22)28-31(23,24)27-29(18,19)20/h1-2,6-8,16H,3-4H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,12,13,14,17)
InChI key
DLLXAZJTLIUPAI-UHFFFAOYSA-N
Application
7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt has been used as a lysis agent to evaluate its efficiency on direct cell lysis, RNA stability, and compatibility with downstream reverse transcription quantitative real-time PCR during the analyzes of samples with small numbers of cells.
Biochem/physiol Actions
7-Deaza-2′-deoxyguanosine 5′-triphosphate (7-deaza-dGTP) competes with natural substrate dGTP and blocks the telomerase activity. This nucleotide once incorporated into the telomere alters the secondary structure of the telomere making it difficult for telomerase to identify it for further telomere synthesis. This dGTP analog pairs weakly with conventional bases to minimize DNA secondary structures, while being a good substrate for DNA polymerases. 7-deaza-dGTP in combination with dimethyl sulfoxide (DMSO) and betaine enhances the polymerase chain reaction (PCR) amplification of GC-rich DNA sequences.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Tatsuo Hata et al.
The Journal of molecular diagnostics : JMD, 20(1), 46-55 (2017-12-13)
Telomere end-to-end fusions are an important source of chromosomal instability that arise in cells with critically shortened telomeres. We developed a nested real-time quantitative PCR method for telomere fusion detection in pancreatic ductal adenocarcinomas, intraductal papillary mucinous neoplasms (IPMNs), and
Andreas Blecha et al.
Microbial cell factories, 4, 28-28 (2005-10-06)
Native as well as recombinant bacterial cell surface layer (S-layer) protein of Geobacillus (G.) stearothermophilus ATCC 12980 assembles to supramolecular structures with an oblique symmetry. Upon expression in E. coli, S-layer self assembly products are formed in the cytosol. We
Russell J Diefenbach et al.
Cancers, 12(8) (2020-08-14)
Detection of melanoma-associated mutations using circulating tumor DNA (ctDNA) from plasma is a potential alternative to using genomic DNA from invasive tissue biopsies. In this study, we developed a custom melanoma next-generation sequencing (NGS) panel which includes 123 amplicons in
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D8783-.5UMO | 04061833589915 |