C8868
Cholesterol Oxidase from microorganisms
lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli
Synonym(s):
Cholesterol: oxygen oxidoreductase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-842-1
MDL number:
Specific activity:
≥50 units/mg protein
Recombinant:
expressed in E. coli
Pricing and availability is not currently available.
recombinant
expressed in E. coli
Quality Segment
form
lyophilized powder
specific activity
≥50 units/mg protein
mol wt
64 kDa
composition
Protein, ≥15% biuret
storage temp.
−20°C
General description
Type II cholesterol oxidase containing FAD cofactor covalently linked to the enzyme.
Application
Cholesterol oxidase has been used in a study to demonstrate that microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. Cholesterol oxidase has also been used in a study that concluded that amphipaths that activate cholesterol might be useful in treating NPC disease.
Cholesterol oxidase is used to determine serum cholesterol.[1][2] The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3β-hydroxysteroids.[3] Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil.[4] CHOD has also been used as a molecular probe to elucidate cellular membrane structures.[5][6]
Biochem/physiol Actions
Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H2O2.[7]
Physical form
Supplied as a lyophilzed powder containing sucrose
Other Notes
One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥50 units/mg protein | specific activity ≥10 units/mg protein | specific activity ≥30 units/mg protein (biuret) | specific activity 8-20 units/mg solid |
| form lyophilized powder | form lyophilized powder | form aqueous solution | form lyophilized powder |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
| mol wt 64 kDa | mol wt 55 kDa | mol wt 62 kDa | mol wt 71 kDa by SDS-PAGE, 83 kDa by gel filtration |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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