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Choline chloride

Name: Choline chloride
Brand: SIGMA

BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%

Synonym(s):

(2-Hydroxyethyl)trimethylammonium chloride

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About This Item

Linear Formula:

(CH₃)₃N(Cl)CH₂CH₂OH

CAS Number:

67-48-1

Molecular Weight:

139.62

UNSPSC Code:

12352205

NACRES:

NA.75

PubChem Substance ID:

24892968

EC Number:

200-655-4

Beilstein/REAXYS Number:

3563126

MDL number:

MFCD00011721

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Properties

biological source

synthetic (organic)

Quality Segment

300

product line

BioReagent

assay

≥98%

form

crystals

mol wt

M_w 139.62 g/mol

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

color

white

pH

5.0-6.5 (25 °C, 140 g/L)

mp

302-305 °C (dec.) (lit.)

solubility

H₂O: 50 mg/mL

SMILES string

[Cl-].C[N+](C)(C)CCO

InChI

1S/C5H14NO.ClH/c1-6(2,3)4-5-7;/h7H,4-5H2,1-3H3;1H/q+1;/p-1

InChI key

SGMZJAMFUVOLNK-UHFFFAOYSA-M

Description

General description

Choline chloride is a quaternary ammonium salt and associates with hydrogen bond donors (HBD). It is essential for the vital physiological functions and is used as a nutritional additive. Choline is a source of methyl groups for methyl-transfer reactions.

Choline acetyltransferase substrate

Application

Choline chloride has been used:

as a component of choline acetyltransferase (ChAT) buffer and for choline uptake in cultured human neuroblastoma (SK-N-SH) cells
for choline standard curve generation for quantifying phospholipase D (PLD) activity
as a component of slice solution for the dissection of entorhinal-hippocampal brain slices

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1 of 1

This Item	26978	26978	W450001
Sigma-Aldrich C7527 Choline chloride	Sigma-Aldrich 26978 Choline chloride	Sigma-Aldrich 26978 Choline chloride	Sigma-Aldrich W450001 Choline chloride
technique(s) cell culture \| insect: suitable, cell culture \| plant: suitable, cell culture \| mammalian: suitable	technique(s) -	technique(s) -	technique(s) -
biological source synthetic (organic)	biological source synthetic	biological source synthetic	biological source synthetic
assay ≥98%	assay ≥99.0% (AT)	assay ≥99.0% (AT)	assay -
form crystals	form powder or crystals	form powder or crystals	form powder, crystals or chunks
solubility H₂O: 50 mg/mL	solubility H₂O: 1 M, clear, colorless	solubility H₂O: 1 M, clear, colorless	solubility -
color white	color colorless to white	color colorless to white	color white
technique(s) cell culture \| insect: suitable, cell culture \| plant: suitable, cell culture \| mammalian: suitable	technique(s) -	technique(s) -	technique(s) -
biological source synthetic (organic)	biological source synthetic	biological source synthetic	biological source synthetic
assay ≥98%	assay ≥99.0% (AT)	assay ≥99.0% (AT)	assay -
form crystals	form powder or crystals	form powder or crystals	form powder, crystals or chunks
solubility H₂O: 50 mg/mL	solubility H₂O: 1 M, clear, colorless	solubility H₂O: 1 M, clear, colorless	solubility -
color white	color colorless to white	color colorless to white	color white

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Questions

1–3 of 3 Questions

a year ago

Choline chloride will be melted in water for addition to the medium. Can you please tell me the proper storage method, such as -30°C freezing ay -4°C refrigeration?

1 answer
1. Technical Support
  
  a year ago
  
  This product is soluble in water at 50 mg/mL. The storage and stability conditions for this product upon reconstitution have not been determined.
  
  Helpful?
a year ago

Hi, is it possible to thaw choline chloride in PBS instead of water and add it to the medium?

1 answer
1. Technical Support
  
  a year ago
  
  This product is soluble in water, tested at 50 mg/mL. Solubility in PBS has not been determined in-house. Literature suggests that choline chloride can be prepared with PBS. For reference, see https://en-cdn.bio-protocol.org/pdf/Bio-protocol807.pdf?rel_link=YmlvLXByb3RvY29sLm9yZy9lbi9icGRldGFpbD9pZD04MDcmdHlwZT0w
  
  Helpful?
3 years ago

Hi, could you please provide the product information sheet for Choline chloride.

1 answer
1. Technical Support
  
  3 years ago
  
  There is no specific product information sheet offered for Choline Chloride. Please see the PROPERTIES section of the product page for details. Links to a sample Certificate of Analysis and a Certificate of Origin are provided below:
  Certificate of Analysis
  https://www.sigmaaldrich.com/certificates/COFA/C7/C7527/C7527-100G________SLBB8921V_.pdf
  Certificate of Origin
  https://www.sigmaaldrich.com/US/en/coo/undefined/C7527/SLBB8921V
  
  Helpful?

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