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A8228

Sigma-Aldrich

Anti-hABH2 antibody, Mouse monoclonal

clone hABH2-7, purified from hybridoma cell culture

Synonym(s):

Anti-Human AlkB Homologue 2, Monoclonal Anti-hABH2 antibody produced in mouse

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About This Item

MDL number:
MFCD06412251
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

hABH2-7, monoclonal

form

buffered aqueous solution

mol wt

antigen ~30 kDa

species reactivity

human

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of 293T cells

isotype

IgG1

UniProt accession no.

Q6NS38

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ALKBH2(121642)

General description

Anti-hABH2 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH2-7 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human hABH2, conjugated to KLH. Human alkB homolog 1, histone H2A dioxygenase (hABH2) shares homology with the AlkB protein in bacteria. hABH2 localizes to the nucleoplasm and occasionally to the nucleoli.

Application

Mouse Monoclonal Anti-hABH2 antibody has been used for western blot assays. The antibody can also be used for IHC, ELISA, microarray, and immunoprecipitation studies.

Biochem/physiol Actions

Removal of the methyl groups of 1-methyladenine (1-meA) and 3-methylcystosine (3-meC) to their unmodified forms is done by human alkB homolog 1, histone H2A dioxygenase (hABH2) and hABH3. hABH2 acts on double stranded DNA.
hABH2 is a Fe (II) dependent dioxygenase involved in the repair of alkylation-induced damage in double stranded DNA. Alterations in hABH2 have been linked to pediatric brain tumor and gastric cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Minimal methylated substrate and extended substrate range of Escherichia coli AlkB protein, a 1-methyladenine-DNA dioxygenase
Koivisto P, et al.
The Journal of Biological Chemistry, 278(45), 44348-44354 (2003)
Reversal of DNA alkylation damage by two human dioxygenases
Duncan T, et al.
Proceedings of the National Academy of Sciences of the USA, 99(26), 16660-16665 (2002)
Wei Gao et al.
Journal of gastroenterology and hepatology, 26(3), 577-584 (2010-12-16)
Methyl or 1, N(6) -ethenoadenine base lesions are frequent and highly-mutagenic or -carcinogenic events in mammalian DNA. Human AlkB homologue-2 (hABH2), a homologue of the Escherichia coli AlkB protein, has been found to be the principal dioxygenase for the repair
Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA
Aas PA, et al.
Nature, 421(6925), 859-859 (2003)
Valentina Cetica et al.
Journal of neuro-oncology, 94(2), 195-201 (2009-03-18)
Alkylating agents, commonly used for brain tumor therapy, induce DNA and RNA lesions that, if not repaired, drive cells to apoptosis. Thus, cellular mechanisms that are responsible for nucleic acid repair are possibly involved in drug resistance. This work analyzes
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