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94890

Sigma-Aldrich

Victoria Blue B

for microscopy (Bact., Hist.)

Synonym(s):

Basic Blue 26, Basic Blue B

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About This Item

Empirical Formula (Hill Notation):
C33H32ClN3
CAS Number:
2580-56-5
Molecular Weight:
506.08
Colour Index Number:
44045
Beilstein:
4094258
EC Number:
219-943-6
MDL number:
MFCD00011878
UNSPSC Code:
12171500
PubChem Substance ID:
57653647
NACRES:
NA.25

grade

for microscopy (Bact., Hist.)

form

powder

mp

206 °C (dec.) (lit.)

SMILES string

[Cl-].CN(C)c1ccc(cc1)/C(=C2/C=C\C(C=C2)=[N+](\C)C)c3ccc(Nc4ccccc4)c5ccccc35

InChI

1S/C33H31N3.ClH/c1-35(2)27-18-14-24(15-19-27)33(25-16-20-28(21-17-25)36(3)4)31-22-23-32(30-13-9-8-12-29(30)31)34-26-10-6-5-7-11-26;/h5-23H,1-4H3;1H

InChI key

LLWJPGAKXJBKKA-UHFFFAOYSA-N

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Application

Victoria Blue B is used as a nuclear stain in cytology/histochemical applications. Victoria Blue B may be used in the quantitative determination of phospholipids.

Signal Word

Danger

Hazard Statements

H302,H314,H317,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1 - Skin Sens. 1

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dye standards, Part II. 4: Victoria blue B (CI 44045). European Committee for Clinical Laboratory Standards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS).
The Histochemical journal, 24(4), 230-232 (1992-04-01)
Yuan-yuan Chen et al.
Guang pu xue yu guang pu fen xi = Guang pu, 26(6), 1148-1150 (2006-09-12)
In pH 4.0 acetic acid-sodium acetate buffer solution, cationic dye victory blue B shows an absorption peak at 614 nm, and the absorption peak decreases after it reacts with chondroitin sulfate to form association particles. The decrease in absorption value
Some variability factors in the cytomorphological analysis of frozen bull semen.
Galli A, Paloschi L, Pizzi F.
Andrologie, 21, 120-126 (1989)
E Schulte et al.
Histochemistry, 88(3-6), 427-433 (1988-01-01)
The aim of this study was to investigate the staining characteristics of Victoria Blue B in alcohol solutions. Cytological specimens (liver and spleen tissue imprints, blood smears) were stained with methanol solutions of commercially available Victoria Blue B-Cl and with
Aikaterini Papadopoulou et al.
Regional anesthesia and pain medicine, 37(2), 183-187 (2012-02-23)
We hypothesized that by substituting a dye surrogate in place of local anesthetic, we could visually demonstrate dye sequestration by lipid emulsion that would be dependent on both dye lipophilicity and the amount of lipid emulsion used. We selected 2
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