Soybean trypsin inhibitor is a mixture of the three types. We have not characterized our trypsin inhibitor products in terms of how much of each of the three types may be present. All three have a molecular weight of between 20,000 and 20,200 daltons, so one might use 20,100 daltons as an average.The three types vary in the amino acids at a few positions. The detailed summary of the three types (or variants) was published in the following linked reference: J. Biochem. (Japan), 98, 435-448 (1985).Note that the authors use Tia, Tib and Tic to indicate the three variants.
93620
Trypsin inhibitor from Glycine max (soybean)
lyophilized powder, ~10000 U/mg
Synonym(s):
SBTI
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About This Item
Pricing and availability is not currently available.
biological source
Glycine max (soybean)
Quality Level
form
lyophilized powder
specific activity
~10000 U/mg
mol wt
Mr ~20000
solubility
0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
Preparation Note
The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Other Notes
For the inhibition of proteolytic activity[1][2]; Use in affinity chromatography for the purification of trypsin; Prepared by the method of M. Kunitz[3].
1 U corresponds to the amount of inhibitor which reduces the trypsin activity by 1 BAEE-U. (1 BAEE-U is the amount of enzyme which increases the absorbance at 253 nm by 0.001 per minute at pH 7.6 and 25 °C; BAEE, Cat. No. 12880, as substrate).
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
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This Item | |||
|---|---|---|---|
| biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) |
| Quality Level 100 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form powder |
| shipped in wet ice | shipped in - | shipped in ambient | shipped in ambient |
| solubility 0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless | solubility H2O: >10 mg/mL, phosphate buffer: >10 mg/mL (hazy above 10 mg/ml), soluble | solubility balanced salt solution: 1 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow, phosphate buffer: 10 mg/mL, water: 10 mg/mL, serum-free medium: soluble | solubility balanced salt solution: 1 mg/mL, serum-free medium: soluble |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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S. Katoh et al.
Polymer Preprints (American Chemical Society, Division of Polymer Chemistry), 21, 94-94 (1980)
The reactive site of trypsin inhibitors.
K Ozawa et al.
The Journal of biological chemistry, 241(17), 3955-3961 (1966-09-10)
Trypsin and chymotrypsin inhibitors from soybeans.
Y Birk
Methods in enzymology, 45, 700-707 (1976-01-01)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 93620-250MG | 04061833283004 |
| 93620-1G | 04061833282991 |
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What is the molecular weight and amino acid sequence of trypsin inhibitor from Glycine max (soybean)?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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