87748

3,3′,5,5′-Tetramethylbenzidine

TMB substrate, chromogenic, ≥98.0% (NT), powder

• Synonym(s): BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
• Linear Formula: [-C₆H₂(CH₃)₂-4-NH₂]₂
• CAS Number: 54827-17-7
• Molecular Weight: 240.34
• NACRES: NA.83
• PubChem Substance ID: 329769606
• UNSPSC Code: 12352204
• EC Number: 259-364-6
• MDL number: MFCD00007748
• Beilstein/REAXYS Number: 2808541

Properties

• Product Name: 3,3′,5,5′-Tetramethylbenzidine, ≥98.0% (NT)
• Quality Level: 300
• assay: ≥98.0% (NT)
• form: powder
• technique(s): UV/Vis spectroscopy: suitable
• mp: 166-171 °C, 168-171 °C (lit.)
• storage temp.: 2-8°C
• SMILES string: Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
• InChI: 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
• InChI key: UAIUNKRWKOVEES-UHFFFAOYSA-N

Description

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

3,3′,5,5′-Tetramethylbenzidine has been used as a substrate for peroxidase in:

• enzyme-immunoassay (EIA)
• indirect competitive enzyme-linked immunosorbent assay (ELISA)
• sandwich enzyme-linked immunosorbent assay

Safety Information

• pictograms: GHS08,GHS07
• signalword: Warning
• hcodes: H302,H351,H413
• pcodes: P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves