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Phosphatase, Alkaline from calf intestinal mucosa

Name: Phosphatase, Alkaline from calf intestinal mucosa
Brand: SIGMA

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

Synonym(s):

Alkaline Phosphatase from calf intestinal mucosa

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About This Item

CAS Number:

9001-78-9

UNSPSC Code:

12352204

eCl@ss:

42010105

EC Number:

232-631-4

NACRES:

NA.54

MDL number:

MFCD00131849

EC Number:

3.1.3.1 (BRENDA, IUBMB)

Specific activity:

~2500 U/mg protein (~10 mg/ml)

Biological source:

bovine (calf) intestinal mucosa

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Properties

biological source

bovine (calf) intestinal mucosa

form

solution (clear, colorless)

specific activity

~2500 U/mg protein (~10 mg/ml)

technique(s)

enzyme immunoassay: suitable

impurities

0.1 mM ZnCl₂, 1 mM MgCl₂, 3 M NaCl, 30 mM triethanolamine

pH

~7.6

storage temp.

2-8°C

Description

Application

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

Preparation Note

The solution can be used without dialysis (for enzyme immuno-assay).

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C

Sales restrictions may apply

Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen[1]; Preparation of enzyme-antibody conjugates[2]; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase[3]

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This Item	P6774	P7923	P0114
Sigma-Aldrich 79390 Phosphatase, Alkaline from calf intestinal mucosa	Sigma-Aldrich P6774 Phosphatase, Alkaline from bovine intestinal mucosa	Sigma-Aldrich P7923 Phosphatase, Alkaline from bovine intestinal mucosa	Sigma-Aldrich P0114 Phosphatase, Alkaline from bovine intestinal mucosa
biological source bovine (calf) intestinal mucosa	biological source bovine intestinal mucosa	biological source -	biological source -
technique(s) enzyme immunoassay: suitable	technique(s) -	technique(s) -	technique(s) -
specific activity ~2500 U/mg protein (~10 mg/ml)	specific activity ≥2,000 DEA units/mg protein	specific activity ≥4,000 DEA units/mg protein	specific activity ≥5,700 DEA units/mg protein
form solution (clear, colorless)	form buffered aqueous solution	form buffered aqueous glycerol solution	form (40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
pH ~7.6	pH -	pH -	pH -

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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