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About This Item
NACRES:
NA.32
UNSPSC Code:
12352108
Quality Segment
assay
≥80.0% (degree of coupling)
fluorescence
λex 637 nm; λem 660 nm±10 nm in water
storage temp.
−20°C
General description
Abberior STAR RED is an already well known dye named also KK114 in literature (a scientific web search shows more than 60 publications, a selection is listed below). Please note the minimal background signal due to negligible unspecific binding. A key feature of the dye is its comparably long lifetime of ~3.5ns. The dye works exceptionally well with the Abberior Instruments STED microscope as well as with the Leica STED microscope.
Abberior STAR RED can substitute Atto™ 647N, AlexaFluor 647, or Cy®5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength around 750 nm is recommended. Please see reference [2] for detailed characteristics.
Best results are obtained with freshly prepared samples.
Abberior STAR RED can substitute Atto™ 647N, AlexaFluor 647, or Cy®5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength around 750 nm is recommended. Please see reference [2] for detailed characteristics.
Best results are obtained with freshly prepared samples.
Application
Abberior® STAR RED labelled cholesterol analogs were used for STED-FCS (stimulated emission depletion - fluorescence correlation spectroscopy) based measurements of membrane diffusion dynamics. Anti-mouse secondary antibody coupled to Abberior® STAR RED has been used for STED microscopy imaging in Vero cell line.
Designed and tested for fluorescent super-resolution microscopy
Other Notes
Legal Information
Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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