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KAPA PROBE FAST One-Step

Name: KAPA PROBE FAST One-Step
Brand: ROCHE

Universal

Synonym(s):

RT-qPCR, probe qPCR, qPCR

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About This Item

NACRES:

NA.55

UNSPSC Code:

41106300

Pricing and availability is not currently available.

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Properties

usage

sufficient for 500 reactions

feature

dNTPs included: no, hotstart: no

packaging

pkg of 500 RXNS

manufacturer/tradename

Roche

technique(s)

RT-qPCR: suitable, whole genome amplification: suitable

input

purified DNA

detection method

probe-based

storage temp.

−20°C

Description

General description

KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal Kit is a sensitive and convenient solution for real-time PCR using RNA as template. The kit is designed for high-throughput, fast-cycling, one-step RNA quantification using sequence-specific fluorogenic probes. It is compatible with all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g. TaqMan^®) and displacement probes (e.g., molecular beacons)

Application

KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal has been used for:

Gene expression analysis
SNP/mutation analysis
Gene knockdown validation
RT-PCR

Features and Benefits

Quick Notes:

The kit is suitable for all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan) and displacement probes (e.g., molecular beacons).
Use only gene-specific primers for one-step qRT-PCR.
Optimal cDNA synthesis is achieved at 42°C for 5 min.
3 min at 95°C is sufficient for RT inactivation and DNA polymerase activation.
Do not exceed 25 μL reaction volumes

Packaging

Kit includes qPCR Master Mix (2X), KAPA RT Mix (50X), dUTP (10 mM), ROX High Reference Dye (50X) and ROX Low Reference Dye (50X)

Preparation Note

Always ensure that components have been fully thawed and thoroughly mixed before use. The KAPA PROBE FAST qPCR Master Mix may not freeze solidly, even when stored at -20 °C. The KAPA RT Mix is temperature-sensitive, and should be stored at -20 °C and kept on ice during use. ROX reference dye is light-sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

Analysis Note

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

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This Item	PFUKB	PFLRKB	PFABIKB
Roche KK4752 KAPA PROBE FAST One-Step	Roche PFUKB KAPA PROBE FAST	Roche PFLRKB KAPA PROBE FAST	Roche PFABIKB KAPA PROBE FAST
description Universal	description suitable for qPCR, 2 ×, Universal	description suitable for qPCR, 2 ×, ROX^™ Low	description suitable for qPCR, 2 ×, ABI Prism^®
technique(s) whole genome amplification: suitable, RT-qPCR: suitable	technique(s) whole genome amplification: suitable, qPCR: suitable	technique(s) whole genome amplification: suitable, qPCR: suitable	technique(s) qPCR: suitable, whole genome amplification: suitable
input purified DNA	input purified DNA	input purified DNA	input -
manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche
packaging pkg of 500 RXNS	packaging kit of 50 mL (5000 x 20 μL rxn; KK4715), kit of 5 mL (500 x 20 μL rxn; KK4702), kit of 10 mL (1000 x 20 μL rxn; KK4703), kit of 1 mL (100 x 20 μL rxn; KK4701)	packaging kit of 1 mL (100 x 20 μL rxn; KK4716), pkg of , kit of 5 mL (500 x 20 μL rxn; KK4717), kit of 10 mL (1000 x 20 μL rxn; KK4718)	packaging kit of 5 mL (500 x 20 μL rxn; KK4706), kit of 10 mL (1000 x 20 μL rxn; KK4707), kit of 1 mL (100 x 20 μL rxn; KK4705), kit of 50 mL (5000 x 20 mL rxn; KK4714), pkg of
usage sufficient for 500 reactions	usage sufficient for 5000 reactions, sufficient for 500 reactions, sufficient for 1000 reactions, sufficient for 100 reactions	usage sufficient for 100 reactions, sufficient for 1000 reactions, sufficient for 500 reactions	usage -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c