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MAB8131

Sigma-Aldrich

Anti-Cytomegalovirus Antibody, immediate early, clone 6F8.2

clone 6F8.2, Chemicon®, from mouse

Synonym(s):

CMV

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6F8.2, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2a

shipped in

wet ice

Specificity

Reacts with an immediate early protein M.W. 68-72 kD. Can detect CMV infection 1 hour post-infection exhibiting a nuclear and/or intranuclear inclusion staining which reaches peak intensity between 8-24 hours.

With CMV the antigens expressed at different times are listed as:



Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD

Immunogen

Affinity purified early antigen from MRC-5 cells infected with CMV AD169 (ATCC).
Epitope: immediate early

Application

Anti-Cytomegalovirus Antibody, immediate early, clone 6F8.2 is an antibody against Cytomegalovirus for use in IF, WB, IC, IH(P).
Immunohistochemistry.

Immunocytochemistry.

Western Blot 1:100

IFA at 1:600-1:1,200 on acetone fixed cells.

Works on paraffin embedded tissue sections.

Final working dilutions must be determined by end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral

Linkage

Replaces: MAB8130

Physical form

Format: Purified
Purified immunoglobulin. Supplied in PBS buffer, pH 7.4. Contains 0.1% sodium azide and carrier protein. Has been sterile filtered.

Storage and Stability

Maintain at +4°C for up to 12 months

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Extensive human cytomegalovirus (HCMV) genomic DNA in the renal tubular epithelium early after renal transplantation: Relationship with HCMV DNAemia and long-term graft function.
Li YT, Emery VC, Surah S, Jarmulowicz M, Sweny P, Kidd IM, Griffiths PD, Clark DA
Journal of Medical Virology null
Dominique McCormick et al.
mBio, 9(3) (2018-06-28)
As obligate intracellular parasites, viruses are completely dependent on host factors for replication. Assembly and egress of complex virus particles, such as human cytomegalovirus (HCMV), are likely to require many host factors. Despite this, relatively few have been identified and
Yao-Tang Lin et al.
PLoS pathogens, 16(9), e1008844-e1008844 (2020-09-05)
The genomes of RNA and small DNA viruses of vertebrates display significant suppression of CpG dinucleotide frequencies. Artificially increasing dinucleotide frequencies results in substantial attenuation of virus replication, suggesting that these compositional changes may facilitate recognition of non-self RNA sequences.
Yan Wang et al.
Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents, 1-9 (2021-01-19)
The AAA+ (ATPase associated with various cellular activities) protein p97, also called valosin-containing protein, is a hexameric ring ATPase and uses ATP hydrolysis to unfold or extract proteins from biological complexes. Many cellular processes are affected by p97 including ER-associated
Mapping the viral genetic determinants of endothelial cell tropism in human cytomegalovirus.
Bolovan-Fritts, Cynthia A and Wiedeman, Jean A
Journal of Clinical Virology, 25 Suppl 2, S97-109 (2002)

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