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Key Documents

AB5745

Sigma-Aldrich

Anti-Opsin Antibody, Red/Green

Chemicon®, from chicken

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

chicken

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Specificity

Opsin, red and green. The antibody reacts with a protein of ~40 kDa.
Other species have not been tested. It is expected that the antibody will also react with zebra finch.

Immunogen

Synthetic peptides from mouse green opsin and zebra finch red opsin.

Application

Detect Opsin using this Anti-Opsin Antibody, Red/Green validated for use in ELISA & WB.
Immunoblotting: 1:500:1:2,000 on mouse brain extract. To minimize background staining it is suggested that you include a higher content of detergent (Tween-20) in your dilution and washing solutions.

Immunohistochemistry: not tested. It is recommended that you try the antibody at 1:50-1:1,000.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Sensory & PNS

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Huirong Li et al.
Molecular neurodegeneration, 16(1), 16-16 (2021-03-12)
Oxidative stress is a common cause of neurodegeneration and plays a central role in retinal degenerative diseases. Heme oxygenase-1 (HMOX1) is a redox-regulated enzyme that is induced in neurodegenerative diseases and acts against oxidative stress but can also promote cell
Patrick W Keeley et al.
Developmental biology, 394(2), 191-196 (2014-08-30)
Programmed cell death contributes to the histogenesis of the nervous system, and is believed to be modulated through the sustaining effects of afferents and targets during the period of synaptogenesis. Cone bipolar cells undergo programmed cell death during development, and

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