420128

JNK Inhibitor II

InSolution, ≥98%

• Synonym(s): InSolution JNK Inhibitor II
• Empirical Formula (Hill Notation): C₁₄H₈N₂O
• Molecular Weight: 220.23
• NACRES: NA.77
• UNSPSC Code: 12352200
• Assay: ≥98% (HPLC)
• Form: liquid
• Storage condition: OK to freeze, desiccated (hygroscopic), protect from light

Properties

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic), protect from light
• color: yellow-brown
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A potent, cell-permeable, selective, and reversible inhibitor of c-Jun N-terminal kinase (JNK) (IC₅₀ = 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3). The inhibition is competitive with respect to ATP. Exhibits over 300-fold greater selectivity for JNK as compared to ERK1 and p38-2 MAP kinases. Inhibits the phosphorylation of c-Jun and blocks the expression of IL-2, IFN-γ, TNF-α, and COX-2 in cells. Blocks IL-1-induced accumulation of phospho-Jun and induction of c-Jun transcription.

Biochem/physiol Actions

Target IC₅₀: 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3

Primary Target
JNK 1, JNK 2, JNK 3

Product competes with ATP.

Reversible: yes

Cell permeable: yes

Packaging

Packaged under inert gas

Physical form

A 50 mM (5 mg/454 µl) solution of JNK Inhibitor II (Cat. No. 420119) in DMSO.

Preparation Note

Following initial use, aliquot and refrigerate (4°C) for short-term storage or freeze (-20°C) for long-term storage.

Other Notes

Bennett, B.L., et al. 2001. Proc. Natl. Acad. Sci. USA98, 13681.
Han, Z., et al. 2001. J. Clin. Invest.108, 73.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: 188.6 °F - closed cup - (Dimethylsulfoxide)
• flash_point_c: 87 °C - closed cup - (Dimethylsulfoxide)