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219374

Cetyltrimethylammonium Bromide, Molecular Biology Grade

Cationic detergent suitable for the isolation of high molecular weight DNA in plants and other organisms.

Synonym(s):

Cetyltrimethylammonium Bromide, Molecular Biology Grade, Cetrimide, CTAB

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About This Item

Empirical Formula (Hill Notation):
C19H42BrN
CAS Number:
Molecular Weight:
364.45
UNSPSC Code:
12161902
NACRES:
NA.21
MDL number:
eCl@ss:
38060102
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grade

Molecular Biology

Quality Segment

description

Merck USA index - 14, 2025

product line

Calbiochem®

assay

≥98% (TLC)

form

crystalline solid

mol wt

364.45 g/mol

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

aggregation number

170

color

white

density

2,30  g/cm3

cation traces

heavy metals (as Pb): ≤0.001%

application(s)

(Isolating DNA)

foreign activity

DNases, proteases, RNases, none detected

shipped in

ambient

storage temp.

15-25°C

SMILES string

[Br-].[N+](CCCCCCCCCCCCCCCC)(C)(C)C

InChI

1S/C19H42N.BrH/c1-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20(2,3)4;/h5-19H2,1-4H3;1H/q+1;/p-1

InChI key

LZZYPRNAOMGNLH-UHFFFAOYSA-M

General description

Cationic detergent suitable for the isolation of high molecular weight DNA in plants and other organisms.

Application


  • Optimization of a high-throughput CTAB-based protocol for the extraction of qPCR-grade DNA from rumen fluid, plant and bacterial pure cultures.: This study details the optimization of a high-throughput protocol using Cetyltrimethylammonium Bromide (CTAB) for extracting high-quality DNA suitable for quantitative PCR (qPCR) from various biological samples. The optimized protocol is significant for molecular biology applications as it ensures efficient and reliable DNA extraction, which is crucial for downstream genetic analyses. (Minas et al., 2011).


Preparation Note

Following reconstitution store at room temprature (20°C). Stock solutions are stable for up to 6 months at room temperature.
Heating to 30-35°C may be required for complete solubilization.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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1 of 1

This Item
137142H626952365
description

Cationic detergent suitable for the isolation of high molecular weight DNA in plants and other organisms.

description

EMPROVE® ESSENTIAL, meets NF

description

Molecular Biology, ≥99%

description

BioUltra, Molecular Biology, ≥99.0% (AT)

grade

Molecular Biology

grade

-

grade

Molecular Biology

grade

Molecular Biology

form

crystalline solid

form

powder

form

powder or crystals

form

powder or crystals

assay

≥98% (TLC)

assay

-

assay

≥99%

assay

≥99.0% (AT)

aggregation number

170

aggregation number

-

aggregation number

170

aggregation number

170

application(s)

(Isolating DNA)

application(s)

downstream processes
pharma/biopharma processes
pharma/biopharma processes

application(s)

-

application(s)

-

storage temp.

15-25°C

storage temp.

2-25°C

storage temp.

-

storage temp.

-


Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2 Oral - STOT SE 3

Target Organs

Gastrointestinal tract, Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

471.2 °F - closed cup

Flash Point (°C)

244 °C - closed cup



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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Can this product be made into a stock solution at around 10-30% w/v and how long will it be stable for?

    1 answer
    1. The solubility in water is listed as being 36.4 g/L at 20°C, or 3.64% w/v. Heating to 30-35°C may be required for complete solubilization and it will be difficult to dissolve at concentrations of 10% or higher. Following reconstitution, it should be stored at room temperature (20°C). Stock solutions are stable for up to 6 months at room temperature.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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