Skip to Content
Merck
All Photos(1)

Key Documents

G005374

HPLC Analysis of 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 on Ascentis® Express F5

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

mobile phase: [A] 7 mM ammonium formate; [B] methanol; (25:75, A:B)
flow rate: 0.4 mL/min
column temp.: 40 °C
detector: UV, 250 nm

ESI(+), TIC m/z 100 - 1000
injection: 1 μL
sample: 300 mg/L in 25:75, water:methanol
column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)

suitability

application for HPLC

application(s)

clinical
vitamins, nutraceuticals, and natural products

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    25-Hydroxyvitamin D3 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
    25-Hydroxyvitamin D2 solution, 50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service