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Key Documents

SAB4502573

Sigma-Aldrich

Anti-C-RAF antibody produced in rabbit

affinity isolated antibody

Synonym(s):

C-RAF, C-Raf, CRAF, Protein kinase raf 1, kinase Raf1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 73 kDa

species reactivity

mouse, human, rat

concentration

~1 mg/mL

technique(s)

ELISA: 1:40000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

P04049

UniProt accession no.

P04049

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAF1(5894)

General description

Anti-C-RAF Antibody detects endogenous levels of total C-RAF protein.
Raf-1 proto-oncogene, serine/threonine kinase (RAF1) or C-RAF is an anti-apoptotic protein. The gene encoding it is localized on human chromosome 3p25.2.

Immunogen

The antiserum was produced against synthesized peptide derived from human C-RAF.

Immunogen Range: 271-320

Biochem/physiol Actions

Raf-1 proto-oncogene, serine/threonine kinase (RAF1) or C-RAF is a crucial component of the Raf/mitogen-activated extracellular signal-regulated kinase (MEK)/extracellular regulated protein kinase (ERK) signaling cascade. It functions as an activator of MEK-1 and -2. The protein is upregulated in many cancers. Mutations in the gene encoding RAF1 have been linked to Noonan syndrome.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The lack of Raf-1 kinase feedback regulation enhances antiapoptosis in cancer cells.
Ma SQ
Oncogene (2017)
Neonatal pulmonary arterial hypertension and Noonan syndrome: two fatal cases with a specific RAF1 mutation.
Hopper RK
American Journal of Medical Genetics (2015)
EZH2-mediated epigenetic repression of DNA repair in promoting breast tumor initiating cells
Breast Cancer Research (2011)
Gain-of-function RAF1 mutations cause Noonan and LEOPARD syndromes with hypertrophic cardiomyopathy
Bhaswati Pandit
Nature Genetics (2007)
Overexpression of Raf-1 and ERK1/2 in sacral chordoma and association with tumor recurrence.
International Journal of Clinical and Experimental Pathology (2015)

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