S0692
Spectinomycin
Ready Made Solution, 100 mg/mL in DMSO/H2O, 1:1
Synonym(s):
Actinospectacin
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About This Item
Recommended Products
biological source
Streptomyces sp.
Quality Level
form
solution
concentration
100 mg/mL in DMSO/H2O, 1:1
color
colorless
solubility
DMSO/H2O, 1:1: soluble 100 mg/mL
antibiotic activity spectrum
Gram-negative bacteria
Gram-positive bacteria
mycobacteria
Mode of action
protein synthesis | interferes
shipped in
wet ice
storage temp.
−20°C
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General description
Chemical structure: aminoglycoside
Application
Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.
Biochem/physiol Actions
Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.
Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).
Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.
Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).
Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.
Caution
Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.
Preparation Note
Stock solutions should be prepared at 10 mg/mL in water and 0.2 μm filtered.
Storage and Stability
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Store under inert gas.
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage. Store under inert gas
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
201.0 °F - closed cup
Flash Point(C)
93.9 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Two new gene cassettes, dfr17 and aadA4, inserted in a class 1 integron of Escherichia coli EC107, are described here. The dfr17 cassette encodes trimethoprim resistance and has 91% identity with the dfrVII dihydrofolate reductase gene. The aadA4 cassette confers
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A P Carter et al.
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The 30S ribosomal subunit has two primary functions in protein synthesis. It discriminates against aminoacyl transfer RNAs that do not match the codon of messenger RNA, thereby ensuring accuracy in translation of the genetic message in a process called decoding.
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
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