N7285
Ninhydrin Reagent
2% Solution
Synonym(s):
1,2,3-Indantrione monohydrate, 2,2-dihydroxyindene-1,3-dione, Ninhydrine
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Application
Ninhydrin reagent has been used to confirm the presence of the amino acid moieties in various reactions.
Ninhydrin reagents can be used in thin layer chromatography (TLC). Ninhydrin reagents have been used to develop and validate a simple, selective, and precise densitometric method for analysis of α-aminocephalosporins, both in bulk drugs and in formulations. Ninhydrin reagents have also been used to observe differences in the reference ranges of several plasma and urine amino acids between Singaporean and Caucasian populations.
Biochem/physiol Actions
Ninhydrin is a tricyclic 1,2,3-trione, which functions as an amino acid reagent. It is a vital organic building block, which exposes latent fingerprints on porous surfaces like paper, cardboard and raw wood. Ninhydrin is used to detect primary and secondary amines. It gives a strong blue color with primary amines and a less intense brown-red color with secondary amine.
Other Notes
Ninhydrin and hydrindantin in DMSO and lithium acetate buffer, pH 5.2
Caution
Store under nitrogen in the refrigerator after use
Certificates of Analysis (COA)
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The analysis of amino acids in plasma and urine was introduced in Singapore when a laboratory for the investigation of inherited metabolic disorders was established by the Ministry of Health. Reference ranges are required for interpreting test results and making
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