pNPP Phosphatase Assay Kit

Name: <I>p</I>NPP Phosphatase Assay Kit
Brand: SIGMA

Sufficient for 500 colorimetric tests

Synonym(s):

Phosphatase Assay Kit, p-Nitrophenyl Phosphate Assay Kit

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About This Item

NACRES:

NA.84

UNSPSC Code:

12161503

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Properties

Quality Segment

100

input

protein

detection method

colorimetric

relevant disease(s)

cancer; hematological disorder; gastrointestinal diseases; neurological disorders; cardiovascular diseases

storage temp.

−20°C

Description

General description

Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at A₄₀₅ nm on a spectrophotometer.

Application

The kit is suitable for the quantitative determination of phosphatase activity of protein phosphatases.

The pNPP Phosphatase Assay Kit may be used for:

Metabolism Research
Cardiovascular Research
Neurodegenerative Research

Biochem/physiol Actions

The pNPP Phosphatase Assay kit involves adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37 °C.

Features and Benefits

Simplified Process: Enjoy a hassle-free procedure that can be completed within 30 minutes without wash or reagent transfer steps, saving you time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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This Item	N4645	P7998	N9389
Sigma-Aldrich MAK461 pNPP Phosphatase Assay Kit	Sigma-Aldrich N4645 4-Nitrophenyl phosphate disodium salt hexahydrate	Sigma-Aldrich P7998 Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA	Sigma-Aldrich N9389 4-Nitrophenyl phosphate disodium salt hexahydrate
Quality Level 100	Quality Level 200	Quality Level 200	Quality Level 300
detection method colorimetric	detection method -	detection method -	detection method -
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
input protein	input -	input -	input -
relevant disease(s) cancer; hematological disorder; gastrointestinal diseases; neurological disorders; cardiovascular diseases	relevant disease(s) -	relevant disease(s) -	relevant disease(s) -