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MAK109

Sigma-Aldrich

LPL Activity Assay Kit

Supplied by Roar Biomedical, Inc.

Synonym(s):

Lipoprotein lipase activity assay kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

application(s)

pharmaceutical

detection method

fluorometric

relevant disease(s)

gastrointestinal diseases; cardiovascular diseases

storage temp.

2-8°C

Gene Information

human ... LPL(4023)

General description

Lipoprotein lipase (LPL) hydrolyzes triglycerides associated with VLDL.

Application

LPL Activity Assay Kit may be used for detection of LPL activity in plasma samples of patients with hypertriglyceridemia.

Suitability

Suitable for the measurement of lipoprotein lipase activity in a variety of tissue samples

Principle

The LPL Activity Assay Kit includes a non-fluorescent substrate emulsion that becomes intensely fluorescent upon interaction with LPL and pre-hydrolyzed substrate for use as a standard to convert the fluorescence intensity reading to moles of reactant formed (λEx=370 nm/λEm=450 nm). The assay is not specific for LPL and will also detect hepatic lipase activity.

related product

Storage Class Code

10 - Combustible liquids


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Maria Notarnicola et al.
Lipids in health and disease, 11, 145-145 (2012-11-01)
Lipid metabolism is altered in subjects with liver steatosis. FAS is a key enzyme in de novo lipogenesis and both FAS gene expression and enzymatic activity are primarily regulated by metabolic signals in the liver. Lipoprotein lipase (LPL), the rate-limiting
Xiaoyao Li et al.
Lipids in health and disease, 17(1), 144-144 (2018-06-21)
Variants in the lipoprotein lipase (LPL), apolipoprotein C-II (APOC2), apolipoprotein A-V (APOA5), GPIHBP1 and LMF1 genes may cause severe hypertriglyceridemia (HTG), which is now the second-leading aetiology of acute pancreatitis in China. The patient and his family were assessed for
Compound but non-linked heterozygous p. W14X and p. L279 V LPL gene mutations in a Chinese patient with long-term severe hypertriglyceridemia and recurrent acute pancreatitis.
Li X, et al.
Lipids in Health and Disease, 17(1), 144-144 (2018)
Evemie Dubé et al.
Biology of reproduction, 88(1), 16-16 (2012-12-12)
Gestational diabetes mellitus (GDM) is a common complication of pregnancy that is characterized by glucose intolerance, leads to dyslipidemia, and is aggravated by obesity. Cholesterol is taken up by the placenta as part of lipoproteins through the scavenger receptor class
Evemie Dubé et al.
Biology of reproduction, 87(1), 14-14 (2012-05-04)
Knowledge of the consequences of maternal obesity in human placental fatty acids (FA) transport and metabolism is limited. Animal studies suggest that placental uptake of maternal FA is altered by maternal overnutrition. We hypothesized that high maternal body mass index

Protocols

Lipoprotein lipase (LPL) hydrolyzes triglycerides associated with VLDL.

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