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Key Documents

G7645

Sigma-Aldrich

Gibberellic acid

suitable for plant cell culture, BioReagent, ≥90% gibberellin A3 basis (of total gibberellins.)

Synonym(s):

GA3, Gibberellin A3

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About This Item

Empirical Formula (Hill Notation):
C19H22O6
CAS Number:
Molecular Weight:
346.37
Beilstein:
54346
EC Number:
MDL number:
UNSPSC Code:
10171502
PubChem Substance ID:
NACRES:
NA.72
Pricing and availability is not currently available.

product line

BioReagent

Quality Level

Assay

≥90% gibberellin A3 basis (of total gibberellins.)

technique(s)

cell culture | plant: suitable

application(s)

agriculture

SMILES string

C[C@]12[C@@H](O)C=C[C@@]3(OC1=O)[C@@H]4CC[C@]5(O)C[C@]4(CC5=C)[C@H]([C@H]23)C(O)=O

InChI

1S/C19H22O6/c1-9-7-17-8-18(9,24)5-3-10(17)19-6-4-11(20)16(2,15(23)25-19)13(19)12(17)14(21)22/h4,6,10-13,20,24H,1,3,5,7-8H2,2H3,(H,21,22)/t10-,11+,12-,13-,16-,17+,18+,19-/m1/s1

InChI key

IXORZMNAPKEEDV-OBDJNFEBSA-N

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General description

Gibberellic acid is a tetracyclic diterpenoid compound, which is synthesized by terpenoid pathway in chloroplasts, cytoplasm, plastid, and endoplasmic reticulum.[1]
Gibberellic acid is an endogenous plant growth regulator

Application

Gibberellic acid has been used as a supplement in Murashige and Skoog media (MS) for treating young leaves of hybrid poplar to follow MYB transcription factor PtrMYB012 expression[2] and in vitro peas seed germination.[3] It has also been used in gibberellic acid (GA)-sensitivity assay in wheat seeds[4] and to promote seed germination in Arabidopsis seeds.[5]
The specific actions of Gibberellin A3 (G1025, G7645) and A4 (G7276) or combinations of Gibberellins should be determined in specific plant applications.

Biochem/physiol Actions

Gibberellic acids (GA) are important plant growth hormones that promote plant cell growth and elongation.[6] Gibberellins promote rapid stem and root growth, induce mitotic division and initiate (break dormancy) and increase seed germination rates.[1] The gibberellins are also involved in processes such as gravitropism and flowering.[7]
Phytohormone that controls important aspects of plant growth: germination, elongation, and flowering. The effects are mediated by inducing degradation of the DELLA repressor protein, and probably other minor signalling pathways.

Preparation Note

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Gibberellic acid in plant: still a mystery unresolved
Gupta R and Chakrabarty, SK
Plant signaling & behavior, 8(9), e25504-e25504 (2013)
Expression patterns of key hormones related to pea (Pisum sativum L.) embryo physiological maturity shift in response to accelerated growth conditions
Ribalta FM, et al.
Frontiers in Plant Science, 10 (2019)
Gibberellic acid production by different fermentation systems using citric pulp as substrate/support
de Oliveira J, et al.
BioMed Research International, 2017 (2017)
Effect of GA-sensitivity on wheat early vigor and yield components under deep sowing
Amram A, et al.
Frontiers in Plant Science, 6, 487-487 (2015)
ICE 1 and ZOU determine the depth of primary seed dormancy in Arabidopsis independently of their role in endosperm development
MacGregor DR, et al.
The Plant Journal, 98(2), 277-290 (2019)

Protocols

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Questions

1–6 of 6 Questions  
  1. How would you recommend that I store a solution of Gibberellic acid, Product G7645?

    1 answer
    1. This product can be initially dissolved in ethanol, diluted with water and stored at 2-8° C.

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  2. Can solutions of Gibberellic acid, Product G7645, be sterilized?

    1 answer
    1. Yes. Sterilization can be either by filter sterilization or coautoclaving with other media components.

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  3. How is the purity of Gibberellic acid, Product G7645, determined?

    1 answer
    1. We measure purity by Thin Layer Chromatography, not by HPLC.

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  4. How do I solubilize Gibberellic acid, Product G7645?

    1 answer
    1. Gibberellic acid is tested for solubility in ethanol (50 mg/ml), yielding a clear, colorless to faint yellow solution.   This may require heating in a water bath for complete solubility.

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  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. What concentration of Gibberellic acid, Product G7645, should I use with my plant culture?

    1 answer
    1. Gibberellic acid is used at 0.01 - 5.0 mg/L.

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