TGP 52 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E) Bri18 mouse. These mice carry a pseudogene construct composed of a pancreatic specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 52 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of pancreatic tumour lines deposited at ECACC. Cells transiently produce insulin in culture followed by somatostatin.
Application
Study of endocrine pancreas carcinomas, hormone assays.
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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ECACC pancreatic cancer cell lines for various applications including drug response studies.
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