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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
NS0
Reactivity
Anti-Factor H, human
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse x Mouse hybridoma
Cell Line Description
The hybridoma line OX-24 was developed by immunizing BALB/c mice with Factor H followed by fusion of spleen cells with NS0 mouse myeloma cells. The antibody was shown to detect the human complement control protein, Factor H. OX-24, which is also known as MRC-OX24 was reported to increase cofactor activity of Factor H in Factor I-mediated cleavage of soluble C3b. The antibody inhibited the binding of radioactive-labelled Factor H to surface-bound C3b. OX-23 (Sigma Catalogue number. 00010402) was shown to decrease this inhibition.
Immunogen
Human Factor H
Culture Medium
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Certificates of Analysis (COA)
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