Skip to Content
Merck
All Photos(1)

Key Documents

H2899

Supelco

HPLC protein standard mixture

analytical standard

Synonym(s):

Protein Calibration Mix

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
85151701
NACRES:
NA.24
Pricing and availability is not currently available.

grade

analytical standard

Quality Level

form

solid

analyte chemical class(es)

amino acids, peptides, proteins

technique(s)

HPLC: suitable

application(s)

food and beverages

format

multi-component solution

storage temp.

−20°C

Application

HPLC protein standard mixture has been used as a standard for the separation of proteins in cell extract samples of bacteria using high performance liquid chromatography (HPLC).[1]

Packaging

Vial contains approximately 1 mg each of ribonuclease A, cytochrome c, holo-transferrin and apomyoglobin.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Honglei Liu et al.
Applied and environmental microbiology, 80(5), 1799-1806 (2014-01-07)
Sulfur oxidation by chemolithotrophic bacteria is well known; however, sulfur oxidation by heterotrophic bacteria is often ignored. Sulfur dioxygenases (SDOs) (EC 1.13.11.18) were originally found in the cell extracts of some chemolithotrophic bacteria as glutathione (GSH)-dependent sulfur dioxygenases. GSH spontaneously
Distribution, diversity, and activities of sulfur dioxygenases in heterotrophic bacteria
Liu H, et al.
Applied and Environmental Microbiology, 80, 1799-1806 (2014)

Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Could you provide an order of retention times of when each of these components elute off a protein C4 column (H2899 protein mixture)?  Thank you.

    1 answer
    1. The exact method is considered proprietary. However, for a C4 column, it should elute ribonuclease A first, followed by cytochrome C, holo-transferrin, and apomyoglobin.

      Helpful?

  4. What is each componant's actual molecular weight (ribonuclease A, cytochrome c, holo-transferrin and apomyoglobin)?

    1 answer
    1. The molecular weight of each component is not reported. See below for the expected values:
      Ribonuclease A: approx. 13.7 kDa
      Cytochrome C: approx. 12.3 kDa
      holo-Transferrin: 72.1 - 78.5 kDa
      Myoglobin: approx. 17.6 kDa

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service