FORMD-RO

Formate Dehydrogenase

from Candida boidinii

• Synonym(s): FDH, Formate:NAD oxidoreductase
• UNSPSC Code: 12352204
• EC Number: 1.2.1.2 (BRENDA, IUBMB)
• NACRES: NA.54
• Specific activity: ~0.4 units/mg protein (At 25 °C with formate as the substrate.)
• Biological source: yeast (Candida boidini)
• Recombinant: expressed in E. coli
• Concentration: ≥10-20 % (w/w)

Properties

• biological source: yeast (Candida boidini)
• recombinant: expressed in E. coli
• form: lyophilized
• specific activity: ~0.4 units/mg protein (At 25 °C with formate as the substrate.)
• mol wt: 74 kDa
• packaging: pkg of 250 U (10837016001), pkg of 80 U (10244678001)
• manufacturer/tradename: Roche
• concentration: ≥10-20 % (w/w)
• technique(s): activity assay: suitable
• color: off-white
• optimum pH: 7.5-8.5
• solubility: water: soluble 10 g/L
• suitability: suitable for gel electrophoresis
• UniProt accession no.: O13437
• application(s): life science and biopharma
• foreign activity: ADH <0.0500%, LDH <0.0500%, MDH <0.1000%
• storage temp.: 2-8°C

Description

General description

Formate:NAD⁺ oxidoreductase
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.

Application

Most widely used in cofactor recycling systems for NADH.

Biochem/physiol Actions

Substrate Specificity and K_m:
Formate dehydrogenase reacts only with formate (K_m = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (K_m 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.

Preparation Note

Store dry

Analysis Note

Contaminants: <0.005% LDH and ADH each, <0.1% MDH

Other Notes

For life science research only. Not for use in diagnostic procedures.

One unit (U) formate dehydrogenase will oxidize 1 μmol of formic acid to CO₂ in 1 minute at +25 °C and pH 7.6. The above assay produces 1 mmol of NADH per mmol of formate oxidized.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash