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MABE1018

Sigma-Aldrich

Anti-BBF2H7/CREB3L2 Antibody, clone 28G9

clone 28G9, from mouse

Synonym(s):

Cyclic AMP-responsive element-binding protein 3-like protein 2, BBF2H7, cAMP-responsive element-binding protein 3-like protein 2/CREB3L2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

28G9, monoclonal

species reactivity

mouse, rat, human

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_848776

UniProt accession no.

Q8BH52

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CREB3L2(64764)

General description

Cyclic AMP-responsive element-binding protein 3-like protein 2 (UniProt Q8BH52; also known as cAMP-responsive element-binding protein 3-like protein 2) is encoded by the Creb3l2 (also known as Bbf2h7, C530025K05Rik, SCIRR69) gene (Gene ID 208647) in murine species. Creb3l2/Bbf2h7 belongs to the old astrocyte specifically induced substance (OASIS) family of ER-resident transcription factors that function as ER stress transducers. OASIS members are ER transmembrane proteins that possess both a transcription-activation domain and a basic leucine zipper (bZIP) domain. Upon initial activation through regulated intramembrane proteolysis (RIP), OASIS family members are transported from the ER to the Golgi apparatus. Sequential cleavage by site-1 protease (S1P) and site-2 protease (S2P) releases the N-terminal fragment, which then translocates to the nucleus and activates the transcription of target genes. Under normal conditions, cellular BBF2H7 and OASIS levels are low due to degradation via the ubiquitin-proteasome pathway as a result of constant E3 ubiquitin ligase HRD1- (HMG-CoA reductase degradation 1-) mediated ubiquitination. ER stress is shown to disrupt their interaction with HRD1, resulting in enhanced stability of BBF2H7 and OASIS and an upregulation of their target genes.

Immunogen

Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of mouse BBF2H7/CREB3L2.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-BBF2H7/CREB3L2 Antibody, clone 28G9 is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of BBF2H7/CREB3L2.
Western Blotting Analysis: 0.25 µg/mL from a representative lot detected BBF2H7/CREB3L2 in 10 µg of mouse MC3T3-E1 preosteoblasts.
Immunoprecipitation: A representative lot co-immunoprecipitated ER-associated E3 Ub ligase Hrd1 with BBF2H7/CREB3L2 from rat C6 glioma cells only before, but not after, ER stress induction by thapsigargin treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).
Western Blottting Analysis: A representative lot detected BBF2H7/CREB3L2 upregulation in murine ATDC5 chondrogenic cells after siRNA-mediated Hrd1 knockdown, as well as BBF2H7/CREB3L2 upregulation in HeLa cells upon proteasome inhibition or ER stress induction (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949.)
Immunocytochemistry: A representative lot detected enhanced ER & nuclear BBF2H7/CREB3L2 immunoreactivity in rat glioma C6 cells upon proteasome inhibitor MG132 treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).

Quality

Evaluated by Western Blotting in rat C6 glioma cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected BBF2H7/CREB3L2 in 10 µg of rat C6 glioma cell lysate.

Target description

~57-60 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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