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MAB5320

Sigma-Aldrich

Anti-Synuclein α Antibody, clone 7B12.2

culture supernatant, clone 7B12.2, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

7B12.2, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SNCA(6622)

Specificity

Reacts with alpha Synuclein. By Western blot the antibody shows no reactivity with beta or gamma synuclein. This antibody was shown to stain Lewy bodies in frozen section of human brain with Parkinson′s disease and glial inclusions in frozen section of human brain with Multiple System Atrophy.

Immunogen

Recombinant human alpha synuclein.

Application

Anti-Synuclein Antibody, α, clone 7B12.2 detects level of Synuclein & has been published & validated for use in IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western blotting on recombinant alpha synuclein: 1:2,000-1:4,000.

Lane 1: Recombinant human alpha synuclein

Lane 2: Recombinant human beta synuclein

Lane 3: Recombinant human gamma synuclein

Immunohistochemistry (frozen sections): 1:250-1:500

Optimal working dilutions must be determined by end user.

Target description

15-18 kDa

Linkage

Replaces: 04-1053

Physical form

UnPurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.
Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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