HTS060M
ChemiScreen Human V 2 Vasopressin Receptor Membrane Preparation
About This Item
biological source
human
Quality Level
recombinant
expressed in Chem-1 cells
form
liquid
packaging
pkg of 4 mL
manufacturer/tradename
ChemiScreen
Chemicon®
technique(s)
ligand binding assay: suitable (GTPγS)
radioligand binding assay (RLBA): suitable
NCBI accession no.
UniProt accession no.
General description
Application
Biochem/physiol Actions
- GPCR Class: A
- Protein Target: V2
- Target Sub-Family: Vasopressin
Specifications
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [3H]-[Arg8] Vasopressin (Perkin Elmer#:NET-800 )
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 8-fold signal:background with 3H labeled [Arg8] Vasopressin at 3.5 nM.
Physical form
Packaging method: Membranes protein were adjusted to 0.5 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C.
Storage and Stability
Legal Information
Disclaimer
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Certificates of Analysis (COA)
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