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AP187B

Sigma-Aldrich

Goat Anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed

1.1 mg/mL, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

biotin conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rabbit

manufacturer/tradename

Chemicon®

concentration

1.1 mg/mL

technique(s)

ELISA: suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

Specificity

Rabbit IgG (H+L) (absorbed for minimal cross-reactivity to Human, Mouse and Rat Serum Proteins)

Application

Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Suggested dilution for most applications:

1:50,000-1:1,000,000 for ELISA using enzyme-conjugated streptavidin

1:10,000-1:100,000 for western blotting with chemiluminescent substrates.

1:500-1:5,000 for enzyme immunoohistochemistry on tissue sections.

1:200-1:1,000 for flow cytometry and fluorescence immunohisto /cytochemistry

Optimal working dilutions must be determined by the end user.
This Goat anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed is validated for use in ELISA, WB for the detection of Rabbit IgG.

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, 0.01% Thimerosal and 0.05% Sodium Azide

RECONSTITUTION:

Reconstitute with 1.5 mL sterile distilled water.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C with or without aliquotting for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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