AB15552

Anti-AT1 Receptor Antibody

Chemicon^®, from rabbit

• Synonym(s): Angiotensin II receptory type 1, AGTR1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, rat, human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI accession no.: NM_000685.4; NM_004835.3; NM_009585.3; NM_031850.2; NM_032049.2
• UniProt accession no.: P30556
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... AGTR1(185)

Description

Specificity

Recognizes AT1 Receptor (Angiotensin II receptory type 1, AGTR1).

The immunogen sequence is identical in canine.

Immunogen

Purified peptide from the N-terminus of human AT1 Receptor (Accession number P30556).

Application

Anti-AT1 Receptor Antibody detects level of AT1 Receptor & has been published & validated for use in FC, IC, IH & WB.

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Hormones & Receptors

Western blot: 1:200-1:500 using ECL on rat liver, rat kidney, mouse heart and mouse kidney lysate.
Immunohistochemistry on rat brain sections.
Immunocytochemistry: 1:100
Flow cytometry, indirect: 5-10 µg of antibody /1 x 106 cells
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified

ImmunoAffinity Purified

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3