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1.07006

Millipore

Oxford Listeria Selective Supplement

selective for Listeria spp., pkg of 10 vials, for use with GranuCult® Oxford Agar (Base)

Synonym(s):

Listeria Selective Supplement

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.74
Pricing and availability is not currently available.

form

solid

Quality Level

200

composition

Acriflavin, 2.5 mg per vial
Cefotetan, 1 mg per vial
Colistin sulfate, 10 mg per vial
Cycloheximide, 200 mg per vial
Fosfomycin, 5 mg per vial

packaging

pkg of 10 vials

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology

compatibility

for use with GranuCult® Oxford Agar (Base)

storage temp.

2-8°C

suitability

selective for Listeria spp.

General description

Oxford Listeria Selective Supplement is a mixture of four antibiotics and a dye in lyophilized form. It inhibits the growth of accompanying bacteria in the selective cultivation of Listeria monocytogenes.

Application

Oxford Listeria Selective Supplement is an additive for the preparation of Oxford Listeria Selective Agar.

Linkage

Replaces: 1.07006.0017

Analysis Note

Supplement tested in Oxford Listeria selective agar (base) art. no. 1.07004.
Recovery on test medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
≥ 70 %
- Listeria monocytogenes ATCC 13932 (WDCM 00021): ≥ 70 %
- Listeria monocytogenes ATCC 35152 (WDCM 00109): ≥ 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %
- Enterococcus faecalis ATCC 19433 (WDCM 00009): ≤ 0.01 %
- Escherichia coli ATCC 25922 (WDCM 00013): ≤ 0.01 %
Black zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
+
- Listeria monocytogenes ATCC 13932 (WDCM 00021): +
- Listeria monocytogenes ATCC 35152 (WDCM 00109): +
- Listeria innocua ATCC 33090 (WDCM 00017): +
Growth
- Staphylococcus aureus ATCC 25923 (WDCM 00034):
inhibited
- Candida albicans ATCC 10231 (WDCM 00054): inhibited
Incubation: 48 hrs.; 35 °C; aerobic.
Reference: Tryptic soy agar.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description
Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Muta. 2 - Repr. 1B

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Certificates of Analysis (COA)

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Articles

Detection of Listeria monocytogenes in Food and Environmental Samples

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

Protocols

Listeria: Surviving Bacteria

Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

Listeria: Surviving Bacteria

Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

Listeria: Surviving Bacteria

Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

Listeria: Surviving Bacteria

Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

Questions

  1. Hello, How can we resuspend the Listeria selective supplement 1070060010 ?

    1 answer

    1. Please below for information on Cat#1.07006.0010

      Preparation
      1. Fill in the bottle of lyophilisate 5 ml mixture 1:1 of ethanol and sterile distilled water and dissolve the lyophilisate. To prepare 500 ml of Oxford-Listeria-Selective-Agar add the dissolved lyophilisate to the sterile culture medium base cooled to a temperature of 50 °C. Mix homogeneously the selective supplement into the culture medium solution by carefully swirling. pH of the ready-to-use medium: 7.0 ± 0.2
      2. Pour the medium into plates and leave to solidify.

      Experimental procedure and evaluation
      Incubate at 37 °C up to 48 h. Listeria monocytogenes produces brown-green coloured colonies with a black halo (esculin splitting). Further biochemical identification must be done.

      Helpful?

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