568223-U

Discovery^® BIO Wide Pore C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm

• Synonym(s): Wide Pore C18 HPLC Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Discovery^® BIO Wide Pore C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
• material: stainless steel column
• Agency: suitable for USP L1
• product line: Discovery^®
• feature: endcapped
• manufacturer/tradename: Discovery^®
• packaging: 1 ea of
• extent of labeling: 9.2% Carbon loading
• parameter: 0-70 °C temperature; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 25 cm × 4.6 mm
• surface area: 100 m²/g
• surface coverage: 3.6 μmol/m²
• matrix: silica particle platform; fully porous particle
• matrix active group: C18 (octadecyl) phase
• particle size: 5 μm
• pore size: 300 Å
• operating pH: 2-8
• separation technique: reversed phase

Description

General description

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Application

• Kinetic performance comparison of superficially porous, fully porous and monolithic reversed-phase columns by gradient kinetic plots for the separation of protein biopharmaceuticals.: This study provides a detailed kinetic performance comparison of different types of reversed-phase HPLC columns, including the Discovery^® BIO Wide Pore C18 HPLC Column. The research highlights its efficiency and resolution in separating complex protein biopharmaceuticals, making it a valuable tool for analytical chemists in biopharmaceutical analysis (Jaag et al., 2022).


• RP-LC x RP-LC analysis of a tryptic digest using a combination of totally porous and partially porous stationary phases.: This paper investigates the use of reverse-phase liquid chromatography (RP-LC) with both totally porous and partially porous stationary phases, including the BIO Wide Pore C18 HPLC Column, to analyze tryptic digests. The findings demonstrate its robustness and high resolution in proteomics applications, enabling precise peptide mapping and protein characterization (Mondello et al., 2010).

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany