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SAB4200708

Sigma-Aldrich

Anti-Proliferating Cell Nuclear Antigen antibody, Mouse monoclonal

clone PC 10, purified from hybridoma cell culture

Synonym(s):

Anti-ATLD2, Anti-Cyclin, Anti-PCNA

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PC 10, monoclonal

form

buffered aqueous solution

mol wt

antigen ~36 kDa

species reactivity

monkey, rat, insect, mouse, yeast, human

concentration

~1.0 mg/mL

technique(s)

ELISA: suitable
flow cytometry: suitable
immunoblotting: 0.5-1 μg/mL using human foreskin fibroblast HS-68 cell line extract
immunofluorescence: 1.25-2.5 μg/mL using HeLa cells
immunohistochemistry: 10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human Tonsil sections.
immunoprecipitation (IP): suitable

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PCNA(5111)

General description

Anti-Proliferating Cell Nuclear Antigen antibody (mouse IgG2a isotype) is derived from the PC 10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. PCNA is an auxiliary protein of DNA polymerase δ and belongs to the conserved sliding clamp family of proteins.
PCNA (proliferating cell nuclear antigen) gene is mapped in human chromosome 20p12.3. PCNA is a homotrimeric protein, which is expressed in the nucleus. The PCNA ring has a large central cavity that allows double-stranded DNA to slide freely along it.

Specificity

Monoclonal Anti-Proliferating Cell Nuclear Antigen (PCNA) specifically recognizes Proliferating Cell Nuclear Antigen from human, monkey, rat, mouse, insect and yeast origin.

Immunogen

PCNA-Protein A fusion protein.

Application

Anti-Proliferating Cell Nuclear Antigen antibody, Mouse monoclonal may be used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • flow cytometry
  • immunohistochemistry
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions

PCNA (proliferating cell nuclear antigen) maintains the polymerase and DNA association by forming a sliding clamp and ensures efficient DNA replication. PCNA is responsible for recruiting important regulatory factors for DNA damage tolerance mechanism. In response to UV irradiation-induced DNA damage, monoubiquitination of PCNA mediates the polymerase switching model by recruiting DNA polymerase η to PCNA. The protein is involved in base excision repair and maintains genome stability. PCNA is associated with proteolysis coupled to DNA synthesis and repair for proper cell cycle progression.
PCNA protein is essential for chromosomal DNA replication during S-phase and is required for several DNA processes like DNA repair, sister chromatid cohesion and cell cycle control. This protein is also involved in the epigenetic modification and chromatin assembly and remodeling. PCNA acts as a prognostic and diagnostic marker in several diseases such as breast cancer, chronic lymphoid leukemia, non-Hodgkin′s lymphoma, malignant and nonmalignant skin diseases and systemic lupus erythematosus.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Replication Protein A Prohibits Diffusion of the PCNA Sliding Clamp along Single-Stranded DNA.
Hedglin M
Biochemistry (2017)
The Functions of Serine 687 Phosphorylation of Human DNA Polymerase ? in UV Damage Tolerance.
Dai X
Molecular and Cellular Proteomics (2016)
PCNA-Dependent Cleavage and Degradation of SDE2 Regulates Response to Replication Stress.
Jo U
PLoS Genetics (2016)
Disruption of PCNA-lamins A/C interactions by prelamin A induces DNA replication fork stalling.
Cobb AM
Nucleus (Austin, Tex.) (2016)
J E Celis et al.
FEBS letters, 220(1), 1-7 (1987-08-10)
Cyclin, also known as PCNA or the auxiliary protein of mammalian DNA polymerase delta, is a stable cell cycle regulated (synthesized mainly in S-phase) nuclear protein of apparent Mr 36,000 whose rate of synthesis correlates directly with the proliferative state

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