R4533
RNAzol® RT
For processing total and small RNA from human, animal, plant, bacterial,and viral samples
Synonym(s):
single step RNA isolation, single step RNA purification, total RNA isolation, total RNA purification
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352200
NACRES:
NA.52
Recommended Products
Quality Level
usage
mL sufficient for 107 cells
mL sufficient for 100 mg tissue (or)
General description
RNAzol RT is a quick and convenient reagent for use in the single-step isolation of total and small RNA from biological samples of human, animal, plant, yeast, bacterial, and viral origin. A convenient single-step liquid phase separation results in the isolation of RNA from DNA, protein, polysaccharides, and other molecules. RNAzol® RT can be used to isolate separate fractions of mRNA and micro RNA or to isolate total RNA, containing all classes of RNA in a single fraction.
This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. Addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can then be removed by centrifugation. RNA can then be isolated by alcohol precipitation, washing and solubilization. Chloroform-induced phase separation is not necessary. One mL of RNAzol® RT is sufficient to isolate RNA from up to 100 mg of tissue or 10e7 cells.
This is one of the most effective methods for isolating total and small RNA and can be completed at room temperature in less than 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types: large nuclear RNA, rRNA, mRNA, small RNA and micro RNA. The resulting RNA is intact with little or no contaminating DNA and protein.
This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. Addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can then be removed by centrifugation. RNA can then be isolated by alcohol precipitation, washing and solubilization. Chloroform-induced phase separation is not necessary. One mL of RNAzol® RT is sufficient to isolate RNA from up to 100 mg of tissue or 10e7 cells.
This is one of the most effective methods for isolating total and small RNA and can be completed at room temperature in less than 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types: large nuclear RNA, rRNA, mRNA, small RNA and micro RNA. The resulting RNA is intact with little or no contaminating DNA and protein.
Application
Isolated RNA can be used for Northern blots, RNase protection assay, microarrays, PCR, and other molecular biology applications.
RNAzol RT is a highly effective reagent for the single-step isolation of total and small RNA from human, animal, plant, bacterial and viral origin samples. The RNA isolation method based on this reagent is performed at room temperature, without the use of chloroform for phase separation, and allows for use in RT-PCR without requiring DNase treatment.
Features and Benefits
- Isolates micro RNA and total RNA isolation of pure and undegraded RNA from biological samples
- High yields and increased quality of isolated RNA
- Completed at room temperature in less than an hour starting with fresh tissue or cells
Legal Information
RNAZol is a registered trademark of Molecular Research Center, Inc.
related product
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
Target Organs
Nervous system,Kidney,Liver,Skin
Storage Class Code
8A - Combustible corrosive hazardous materials
WGK
WGK 3
Flash Point(F)
230.0 °F
Flash Point(C)
110 °C
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Andrea Miccoli et al.
Marine environmental research, 127, 137-147 (2017-04-17)
Natural/synthetic Endocrine Disrupting Chemicals (EDCs) may display estrogenic activity and a lower potency than 17β-estradiol. Nonetheless, their concentrations and additive effects can affect the endocrine system and reproductive processes related to the Hypothalamic-Pituitary-Gonadal (HPG) axis. Because of their persistence in
The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens.
Antonissen G
PLoS ONE, 9(9), e109775-e109775 (2014)
Bryan A Martinez et al.
eLife, 9 (2020-02-26)
In the nematode C. elegans, insulin signaling regulates development and aging in response to the secretion of numerous insulin peptides. Here, we describe a novel, non-signaling isoform of the nematode insulin receptor (IR), DAF-2B, that modulates insulin signaling by sequestration
Barbara Ahlemeyer et al.
Methods and protocols, 3(2) (2020-05-28)
In transfection experiments with mammalian cells aiming to overexpress a specific protein, it is often necessary to correctly quantify the level of the recombinant and the corresponding endogenous mRNA. In our case, mouse calvarial osteoblasts were transfected with a vector
V Shilova et al.
Cell stress & chaperones, 25(2), 305-315 (2020-02-11)
Here, we monitored the expression of three genes (hsp70, hsp22, and hsf1) involved in heat shock response in Drosophila melanogaster in males and females of different age. Also, we investigated age- and sex-dependent expression of three major genes participating in
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service