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D0571

Sigma-Aldrich

Monoclonal Anti-DICER1 antibody produced in mouse

~1.0 mg/mL, clone DCR1, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-DCR1, Anti-HERNA, Anti-Helicase with RNASE motif, Anti-Helicase-MOI, Anti-K12H4.8-LIKE

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DCR1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~218 kDa

species reactivity

human, mouse, rat

concentration

~1.0 mg/mL

technique(s)

western blot: 2.5-5.0 μg/mL using whole extract of human HEK-293T cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

Dicer is a member of the RNase III family containing an N-terminal DEXH-box RNA helicase/ATPase domain, followed by a domain of unknown function (DUF283), a PAZ domain, which anchors the 3′-end of the guided siRNA, two RNase III domains, and a dsRBD. The current model for dsRNA cleavage by Dicer predicts that the two ribonuclease III domains of Dicer dimerize to form the catalytic center that is responsible for cleaving long dsRNA in co-operation with two RNA binding domains, PAZ and dsRNA-binding domain.
Monoclonal Anti-DICER1 (mouse IgG1 isotype) is derived from the hybridoma DCR1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to a fragment of human DICER1.

Application

The antibody may be used in several immunological techniques including immunoblotting and real‐time PCR.

Biochem/physiol Actions

Dicer catalyzes the first step in the RNA interference (RNAi) pathway and initiates formation of the RNA-induced silencing complex (RISC). The dsRNA is processed into small fragments called short interfering RNA (siRNA) or microRNA (miRNA) of typically 21-25 nucleotides long with a two-base overhang on the 3′ end. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. DICER1-deficient colorectal cancer cells have an enhanced ability to initiate tumors and metastasis. Dicer functions as an indirect tumor suppressor, as silencing Dicer expression makes cancer cells more malignant.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yukihide Tomari et al.
Genes & development, 19(5), 517-529 (2005-03-03)
RNA silencing pathways convert the sequence information in long RNA, typically double-stranded RNA, into approximately 21-nt RNA signaling molecules such as small interfering RNAs (siRNAs) and microRNAs (miRNAs). siRNAs and miRNAs provide specificity to protein effector complexes that repress mRNA
Richard I Gregory et al.
Cell, 123(4), 631-640 (2005-11-08)
RNA interference is implemented through the action of the RNA-induced silencing complex (RISC). Although Argonaute2 has been identified as the catalytic center of RISC, the RISC polypeptide composition and assembly using short interfering RNA (siRNA) duplexes has remained elusive. Here
Gunter Meister et al.
Nature, 431(7006), 343-349 (2004-09-17)
Double-stranded RNA (dsRNA) is an important regulator of gene expression in many eukaryotes. It triggers different types of gene silencing that are collectively referred to as RNA silencing or RNA interference. A key step in known silencing pathways is the
Shuguang Zeng et al.
Oncology reports, 31(2), 867-873 (2013-12-10)
microRNAs (miRNAs) are aberrantly expressed in cancer. An enzyme essential for miRNA processing is Dicer, whose expression is deregulated in diverse types of cancer and correlates with tumor progression. However, whether the regulation of Dicer expression affects tongue squamous cell
Critical role of the miR-200 family in regulating differentiation and proliferation of neurons
Pandey A, et al.
Journal of Neurochemistry, 133(5), 640-652 (2015)

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