CA201
cAMP Enzyme Immunoassay Kit
sufficient for 96 assays
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Technique(s):
ELISA: suitable
Species reactivity:
-
Application:
ELISA
Citations:
25
General description
Non-radioactive, competitive immunoassay for the quantitation of cAMP in biological fluids (serum, plasma, and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cAMP to competitively bind cAMP or cAMP which has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cAMP present in the well.
Application
cAMP Enzyme Immunoassay Kit has been used to quantify the cAMP level.
Other Notes
Please refer to the attached Protocolfor details.
Kit Components Only
Product No.
Description
- Acetic Anhydride 1 x 1
- Assay Buffer 2 1 x 30
- cAMP-Alkaline Phosphatase Conjugate 1 x 5
- cAMP Assay Layout Sheet 1 x 1
- cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5
- 5 Cycle Logit-Log Paper 1 x 1
- Cyclic AMP Standard 1 x 0.5
- Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
- p-Nitrophenyl Phosphate Substrate Solution 1 x 20
- Plate Sealer 1 ea
- Stop Solution 1 x 5
- Triethylamine 1 x 2
- Wash Buffer Concentrate 1 x 30
See All (13)
signalword
Danger
target_organs
Respiratory system
Storage Class
3 - Flammable liquids
wgk
WGK 3
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1A - STOT SE 3
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Jonathan Mowers et al.
eLife, 2, e01119-e01119 (2013-12-26)
Obesity produces a chronic inflammatory state involving the NFκB pathway, resulting in persistent elevation of the noncanonical IκB kinases IKKε and TBK1. In this study, we report that these kinases attenuate β-adrenergic signaling in white adipose tissue. Treatment of 3T3-L1
The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae
Kalivoda EJ, et al.
Applied and Environmental Microbiology, 74(11), 3461-3470 (2008)
Abstracts of the XXIII Congress of the International Society on Thrombosis and Haemostasis with the 57th Annual SSC (Scientific and Standardization Committee) Meeting. July 23-28 2011. Kyoto, Japan.
Journal of thrombosis and haemostasis : JTH, 9 Suppl 2, 1-1055 (2011-08-10)
Role of beta3-adrenoceptors for intrahepatic resistance and portal hypertension in liver cirrhosis
Trebicka J, et al.
Hepatology, 50(6), 1924-1935 (2009)
Lingyue Yuan et al.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 40(10), 1465-1471 (2020-10-30)
To elucidate the pathogenic role of leukotriene B4 (LTB4) in pulmonary hyper-permeability and inflammation induced by lung-protective mechanical ventilation (LPMV) in rabbits. Thirty-two healthy Japanese white rabbits were randomized into 4 groups for treatment with vehicle or bestatin (a leukotriene
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Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CA201-1KT | 04061834254324 |
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