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C8696

Sigma-Aldrich

Cathepsin D from human liver

lyophilized powder, ≥250 units/mg protein (E1%/280)

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32

form

lyophilized powder

Quality Level

specific activity

≥250 units/mg protein (E1%/280)

mol wt

~45 kDa

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... CTSD(1509)

General description

Cathepsin D is an aspartic protease, which is located in lysosomes. It is involved in protein catabolism and maintains hormone and antigen processing. Cathepsin D is implicated in neoplasia and neurodegenerative changes. It regulates lysosomal proteolysis and endogenous fibrinolysis.

Application

Cathepsin D from human liver has been used:
  • in β-secretase activity assay
  • for enzymatic degradation of amyloid β 1-42
  • in microinjection of human foreskin fibroblasts

Biochem/physiol Actions

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.

Other Notes

Contains α, β and γ isoenzymes as observed by isoelectric-focusing.

Unit Definition

One unit will produce an increase in A280 of 1.0 in 30 min at pH 3.3 at 37 °C measured as TCA-soluble products using acid-denatured hemoglobin as substrate (1 cm light path).

Physical form

Powder containing sodium phosphate buffer salt.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Platelet membrane beta-secretase activity in mild cognitive impairment and conversion to dementia: a longitudinal study
McGuinness B, et al.
Journal of Alzheimer'S Disease, 49(4), 1095-1103 (2016)
Aspartic Proteinases Physiology and Pathology (1995)
Jeane do Nascimento Moraes et al.
The journal of venomous animals and toxins including tropical diseases, 28, e20220002-e20220002 (2022-11-22)
Cathepsin D (CatD) is a lysosomal proteolytic enzyme expressed in almost all tissues and organs. This protease is a multifunctional enzyme responsible for essential biological processes such as cell cycle regulation, differentiation, migration, tissue remodeling, neuronal growth, ovulation, and apoptosis.
Jason S King et al.
Molecular biology of the cell, 24(17), 2714-2726 (2013-07-26)
Wiskott-Aldrich syndrome protein and SCAR homologue (WASH) is an important regulator of vesicle trafficking. By generating actin on the surface of intracellular vesicles, WASH is able to directly regulate endosomal sorting and maturation. We report that, in Dictyostelium, WASH is
Microinjection of cathepsin d induces caspase-dependent apoptosis in fibroblasts
Roberg K, et al.
The American Journal of Pathology, 161(1), 89-96 (2002)

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