Glycobiology - Carbohydrate Enzymes and Proteins
Explore our complex carbohydrate proteins, enzyme products and kits for investigating oligosaccharides, polysaccharides, and numerous additional aspects of your carbohydrate workflow.
Complex Carbohydrates |
||
| Enzyme Index Agarase Alginate Lyase α-Amylase β-Amylase Amyloglucosidase Cellulase Chitinase Chitosanase Chondroitinases Dextranase Driselase α-Glucosidase Hemicellulase Heparinases Hyaluronidase Inulinase Isoamylase Lysozyme Lyticase Pectinase Pectolyase Pectinesterase Pullulanase Xylanase |
Carbohydrate Index Agarose Alginic Acid Amylose Amylopectin Cellulose Chitin Chitosan Chondroitin Sulfates Dextran β-Glucans Glycogen Hemicellulose Heparan Sulfate Heparin Hyaluronic Acid Inulin Lichenen Pectins Peptidoglycans Pullulan Starch Xylan |
Kits and Reagents for Glycoprotein Analysis Glycoprotein Analysis Manual |
Agarose
Agarose is the principal neutral gelling component of agar extracted from algae. Agarose is a complex range of polysaccharide chains composed of alternating α-(1-3)-D-galactosyl-β-(1-4)-anhydro-L-galactosyl units.1
Agarase
EC# 3.2.1.81
Synonym: agarose 3-glycanohydrolase
Agarase catalyzes the hydrolysis of 1,3-b-D-galactosidic linkages in agarose, giving the tetramer as the predominant product. 1
from Pseudomonas atlantica
Product Number A6306 (lyophilized powder, ≥5,000 units/mg protein (Lowry)
References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/81)
Composed of blocks of β-D-(1-4) mannuronic acid homopolymeric regions (MMMM…), α-L-(1-4)guluronic acid (GGGG…) homopolymeric regions, and alternating copolymer regions of β-D-(1-4) mannuronic acid - α-L-(1-4)-guluronic acid (GMGMGM…). Bacterial alginic acid can be acetlylated at the 2 or 3 positions on mannuronic acid.
Alginate Lyase
EC# 4.2.2.3
Synonyms: poly(β-D-manuronate) lyase
Alginate lyase cleaves at the β-(1-4)-D-mannuronic bonds residues to yield oligosaccharides with 4-deoxy-α-L-erythro-hex-4-enopyranuronosyl groups at their non-reducing termini.
from Flavobacterium
Product Number A1603 (powder, ≥10,000 units/g solid)
References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC4/2/2/3.html)
Polymers of α-(1-4)-D-glycopyranosyl units. Amylopectin has approximately 4% α-(1-6)-D-glycopyranosyl branching. Glycogen tends to have a higher frequency of branches of shorter length than amylopectin.
ɑ-Amylase
EC# 3.2.1.1
Synonyms: 1,4-alpha-D-glucan glucanohydrolase
α-Amylase catalyzes the endohydrolysis of 1,4-α-D-glucosidic linkages in polysaccharides containing three or more 1,4-α-linked D-glucose units.
from Bacillus species
Product Number A6814 (powder, ≥400 units/mg protein (Lowry))
Product Number A6380 (Type II-A, lyophilized powder, ≥1,500 units/mg protein (biuret))
from Bacillus amyloliquefaciens
Product Number A7595 (liquid, ≥250 units)
from Bacillus licheniformis
Product Number A4551 (lyophilized powder, 500-1,500 units/mg protein, 93-100% (SDS-PAGE))
Product Number A3403 (Type XII-A, saline solution, ≥500 units/mg protein (biuret))
Product Number A3306 (solution, for use in Total Dietary Fiber Assay, TDF-100A)
from Aspergillus oryzae
Product Number 10065 (powder, ~30 U/mg)
Product Number 86250 (powder, ~1.5 U/mg (~0.2 U acc. to Willstätter)
Product Number A9857 (≥150 units/mg protein (biuret)
Product Number 86247 (powder, slightly beige, ~100 U/mg)
Product Number A8220 (aqueous solution, ≥800 FAU/g)
from Barley malt
Product Number A2771 (min 1 un/mg, lyophilized powder containing lactose)
from Porcine pancreas
Product Number A3176 (Type VI-B, ≥5 units/mg solid)
Product Number A6255 (DFP Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280))
Product Number A4268 (Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280)
from Human pancreas
Product Number A9972 (lyophilized powder, ≥100 units/mg protein)
from Human saliva
Product Number A1031 (Type XIII-A, lyophilized powder, 300-1,500 units/mg protein)
Product Number A0521 (Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein)
References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/1.html/)
β-Amylase
EC# 3.2.1.2
Synonyms: 1,4-α-D-glucan maltohydrolase
β-Amylase catalyzes the exo-hydrolysis of 1,4-α-D-glucosidic linkages in polysaccharides resulting in the successive liberation of maltose units from the non-reducing ends of the chains.
from Barley
Product Number A7130 (Type II-B, 20-80 units/mg protein (biuret))
from Sweet potato
Product Number A8781 (Gel filtration molecular weight marker, vial of 15 mg)
Product Number A7005 (Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280))
References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/2.html/
Amyloglucosidase
EC# 3.2.1.3
Synonyms: glucan 1,4-α-glucosidase, glucoamylase, γ-amylase
Amyloglucosidase catalyzes the hydrolysis of terminal 1,4-linked α-D-glucose residues successively from the non-reducing ends of maltooligo- and polysacharides with release of β-D-glucose. Most forms of the enzyme can rapidly hydrolyze 1,6-α-D-glucosidic bonds when the next bond in the sequence is 1,4- and some preparations of this enzyme hydrolyze 1,6- and 1,3-α-D-glucosidic bonds in other polysaccharides.
from Aspergillus niger
Product Number 10115 (lyophilized, powder, ~70 U/mg)
Product Number 10113 (powder, white, ~120 U/mg)
Product Number A7420 (lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose)
Product Number A7095 (≥260 U/mL, aqueous solution)
Product Number A1602 (ammonium sulfate suspension, ≥40 units/mg protein)
Product Number ROAMYGLL (Lyophilized powder, approximately 6 U/mg lyophilizate)
Product Number ROAMYGL (Ammonium sulfate suspension, approximately 14 units/mg protein)
from Rhizopus species
Product Number A9228
References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/3.html/)
α-Glucosidase
EC# 3.2.1.20
Synonyms: maltase
α-Glucosidase catalyzes the hydrolysis of terminal 1,4-linked α-D-glucose residues successively from the non-reducing ends of maltooligo- and to a lesser extent polysacharides with release of β-D-glucose. Most forms of the enzyme can slowly hydrolyze 1,6-α-D-glucosidic bonds.
from Bacillus stearothermophilus
Product Number G3651 (lyophilized powder, ≥50 units/mg protein)
from Saccharomyces cerevisiea
Product Number G0660 (recombinant, expressed in proprietary host, lyophilized powder, ≥100 units/mg protein)
Product Number G5003 (Type I, lyophilized powder, ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
from Rice
Product Number G9259 (Type V, ammonium sulfate suspension, 40-80 units/mg protein)
References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/20.html/)
Isoamylase
EC# 3.2.1.68
Synonyms: glycogen a-1,6-glucanohydrolase, debranching enzyme
from Pseudomonas sp.
Product Number 8124 (ammonium sulfate suspension, ≥10,000,000 units/mg protein)
Isoamylase catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/68.html/)
Repeating linear polymer of two β-(1-4)-D-glycopyranosyl and one β-(1-3)-D-glycopyranosyl unit.
Polymer of β-(1-4)-D-glycopyranosyl units occurring as predominantly as cellotriose and cellotetraose separated by single β-(1-3)-D-glycopyranosyl units. Cross-linking can occur within the consecutive cellotriose regions.
Polymer of β-(1-3)-D-glycopyranosyl units with branching at β-(1-6)-D-glycopyranosyl units.
Cellulase
EC# 3.2.1.4
Synonyms: 1,4-(1,3;1,4)-β-D-glucan 4-glucanohydrolase
from Aspergillus species
Product Number C2605 (aqueous solution)
from Aspergillus niger
Product Number 22178 (powder, off-white, ~0.8 U/mg)
Product Number C1184 (powder, ≥0.3 units/mg solid)
from Trichoderma reesei
Product Number C8546 (lyophilized powder, ≥1 unit/mg solid)
Product Number C2730 (aqueous solution, ≥700 units/g)
from Trichoderma viride
Product Number C0615 (powder, ≥5,000 units/g solid)
Product Number C1794 (BioReagent, suitable for plant cell culture, 3-10 units/mg solid)
Product Number C9422 (powder, min. 3.0-10 un/mg)
Cellulase catalyzes the endohydrolysis of 1,4-β-D-glucosidic linkages in cellulose, lichenin and cereal β-D-glucans1
Cellobiohydrolase I
from Hypocrea jecorina.
Product Number E6412 (0.13 U/mg, recombinant, expressed in corn)
Driselase
from Basidiomycetes sp.
Product Number D9515 (powder, Protein ≥10 % by biuret)
Laminarinase
EC# 3.2.1.6
Synonyms: endo-1,3(4)-β-glucanase
Laminarinase catalyzes the endohydrolysis of 1,3- or 1,4-linkages in β-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolysed is itself substituted at C-3.
from Penicillium Species
Product Number L9295 (lyophilized powder, 5-10 un/mg)
Lyticase
Synonyms: Zymolyase
from Arthrobacter luteus
Product Number SAE0098 (free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder)
Product Number L2524 (lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret)
Product Number L4025 (lyophilized powder, ≥200 units/mg solid)
Product Number SAE0098 (lyophilized powder, min 4,000 un/mg protein)
To continue reading please sign in or create an account.
Don't Have An Account?