W0138
Western Blocker™ Solution
for HRP detection systems
Synonym(s):
Western blot blocking solution, blocking buffer
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400 ML
$169.00
$169.00
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About This Item
NACRES:
NA.25
UNSPSC Code:
41105329
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This Item | |||
|---|---|---|---|
| shelf life ≥2 yr | shelf life - | shelf life - | shelf life - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. - |
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level - |
![Western Blocking Reagent, Solution solution, sufficient for 10 blots (11921673001 [100 cm2]), sufficient for 60 blots (11921681001 [100 cm2])](/deepweb/assets/sigmaaldrich/product/images/352/091/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1/640/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1.jpg)
General description
Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.
Application
Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes.[1][2] It has also been used for dilution of antibodies in western blot.[3]
Western Blocker Solution has been used for blocking of western blot membranes.[1][2] It has also been used for dilution of antibodies in western blot.[3]
Features and Benefits
- Does not inhibit weak HRP signals, as many milk-based blockers do.
- Can be used both as a blocking component and as a probing component.
- Supplied ready-to-use; no preparative step is required.
Legal Information
Western Blocker is a trademark of Sigma-Aldrich Co. LLC
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
Hazard Classifications
ED ENV 1
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Danger
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| W0138-400ML | 04061838260512 |
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