This product may be reconstituted in water to a concentration of 0.1-1.0 mg/ml. Please see the link below to review additional information available in the product datasheet:
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V7259
Vascular Endothelial Growth Factor human
≥98% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym(s):
VEGF
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Product Name
Vascular Endothelial Growth Factor human, VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture
biological source
human
recombinant
expressed in E. coli
assay
≥98% (SDS-PAGE)
form
powder
potency
1-10 ng/mL ED50/EC50
quality
endotoxin tested
mol wt
protein 38.2 kDa (as homodimer)
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤0.1 EU/μg
color
white
solubility
water: soluble
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... VEGFA(7422)
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This Item | V3388 | V4512 | P3076 |
|---|---|---|---|
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| assay ≥98% (SDS-PAGE) | assay ≥97% (SDS-PAGE) | assay ≥98% (SDS-PAGE) | assay ≥98% (SDS-PAGE and HPLC) |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source human | biological source human | biological source mouse | biological source human |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form powder | form lyophilized powder | form powder | form lyophilized powder |
Application
Biochem/physiol Actions
Vascular Endothelial Growth Factor (VEGF), also known as vasculotropin, is an angiogenic growth factor, which is heat and acid stable. VEGFs stimulate endothelial cell growth, angiogenisis, and capillary permeability. VEGF is a secreted homodimeric, heparin-binding glycoprotein,1 which has an isoelectric point of 8.5. VEGF promotes the growth of endothelial cells isolated from bovine adrenal cortex, cerebral cortex, fetal and adult aorta, and human umbilical vein. The target cell specificity of VEGF is restricted to vascular endothelial cells. VEGF has no mitogenic effect on cultured corneal endothelial cells, vascular smooth muscle cells, BHK-12 fibroblasts, keratinocytes, human sarcoma cells, or lens epithelial cells. A variety of human tumor cell lines including sarcoma and carcinoma cells show a 3.7 kb RNA transcript that hybridizes with the VEGF probe in a Northern blot. Mouse sarcoma 180 cells express the VEGF mRNA and secrete a VEGF-like mitogen.
VEGF recombinant protein might have potential as a therapeutic agent in treatment-resistant disorders, where it can facilitate endothelial cell growth and vessel formation.[4]
VEGF recombinant protein might have potential as a therapeutic agent in treatment-resistant disorders, where it can facilitate endothelial cell growth and vessel formation.[4]
General description
Monomer consists of 165 amino acid residues, approx. mol. weight 19.2 kDa
Vascular Endothelial Growth Factor (VEGF) gene is localized to human chromosome 6p21.3, and is thought to encode four different isoforms.[4]
Physical form
Lyophilized from a sterile filtered buffered solution with no additives.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Behrous Davani et al.
Stem cells (Dayton, Ohio), 25(12), 3215-3222 (2007-09-29)
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The biology of vascular endothelial growth factor.
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Endocrine reviews, 18(1), 4-25 (1997-02-01)
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Heterotopic and orthotopic ovarian tissue autotransplantation techniques, currently used in humans, will become promising alternative methods for fertility preservation in domestic and wild animals. Thus, this study describes for the first time the efficiency of a heterotopic ovarian tissue autotransplantation
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The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(29), 10666-10670 (2011-07-22)
Originally identified as an angiogenic factor, vascular endothelial growth factor (VEGF-A) is now known to play multiple roles in the CNS, including the direct regulation of neuronal and astrocytic functions. Here, we ask whether VEGF-A can also have a novel
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Instructions
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