Soybean trypsin inhibitor is a mixture of the three types. We have not characterized our trypsin inhibitor products in terms of how much of each of the three types may be present. All three have a molecular weight of between 20,000 and 20,200 daltons, so one might use 20,100 daltons as an average.The three types vary in the amino acids at a few positions. The detailed summary of the three types (or variants) was published in the following linked reference: J. Biochem. (Japan), 98, 435-448 (1985).Note that the authors use Tia, Tib and Tic to indicate the three variants.
T6522
Trypsin inhibitor from Glycine max (soybean)
powder, BioReagent, suitable for cell culture
Trypsin inhibitor from Glycine max (soybean)
Synonym(s):
SBTI
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25 MG
$95.70
100 MG
$204.00
250 MG
$424.00
500 MG
$797.00
5 X 100 MG
$897.00
1 G
$932.00
5 G
$4,600.00
About This Item
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Product Name
Trypsin inhibitor from Glycine max (soybean), powder, BioReagent, suitable for cell culture
storage temp.
2-8°C
Quality Level
biological source
Glycine max (soybean)
type
Type I-S
product line
BioReagent
form
powder
specific activity
(One mg will inhibit 1.0-3.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.)
mol wt
20.1 kDa
concentration
10 mg/mL
technique(s)
cell culture | mammalian: suitable
solubility
balanced salt solution: 1 mg/mL
serum-free medium: soluble
shipped in
ambient
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This Item | T9128 | SRE0044 | T9767 |
|---|---|---|---|
| biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) |
| technique(s) cell culture | mammalian: suitable | technique(s) inhibition assay: suitable | technique(s) cell culture | mammalian: suitable | technique(s) electrophoresis: suitable |
| specific activity (One mg will inhibit 1.0-3.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.) | specific activity - | specific activity - | specific activity - |
| concentration 10 mg/mL | concentration - | concentration - | concentration - |
| form powder | form lyophilized powder | form lyophilized powder | form powder |
| shipped in ambient | shipped in ambient | shipped in wet ice | shipped in ambient |
Analysis Note
One mg of trypsin inhibitor will inhibit 1.0-3.0 mg trypsin of activity ~10,000 BAEE units per mg protein.
Application
This product has been tested for cell culture applications. Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a ton-toxigenic mangrove isolate of Vibri sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin.
Biochem/physiol Actions
This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
Other Notes
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.
The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.
Preparation Note
The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
This technical article described the Enzymatic Assay of Trypsin Inhibitor.
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
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