If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
T4661
Base Trizma®
≥99.9% (titration), crystalline
Synonyme(s) :
2-Amino-2-(hydroxyméthyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxyméthyl)aminométhane, Trométamol
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494,00 $
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A propos de cet article
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Description
aminopeptidase substrate
Niveau de qualité
Essai
≥99.9% (titration)
Forme
crystalline
Perte
≤5% (loss on drying, 110 °C)
Couleur
white
pH
10.0-11.5
Plage de pH utile
7-9
pKa (25 °C)
8.1
pb
219-220 °C/10 mmHg (lit.)
Pf
167-172 °C (lit.)
Solubilité
water: soluble (400 g plus 600 mL of water)
λ
(290nm < 0.05)
in 5 mM Tris; DNA (40% (w/w))
Application(s)
diagnostic assay manufacturing
Chaîne SMILES
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
Clé InChI
LENZDBCJOHFCAS-UHFFFAOYSA-N
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1 of 4
Cet article | T1503 | T6791 | T6066 |
|---|---|---|---|
| assay ≥99.9% (titration) | assay ≥99.9% (titration) | assay ≥99.9% (titration) | assay ≥99.9% (titration) |
| Quality Level 400 | Quality Level 300 | Quality Level 400 | Quality Level 400 |
| form crystalline | form crystalline | form crystalline | form crystalline powder |
| application(s) diagnostic assay manufacturing | application(s) cell analysis | application(s) diagnostic assay manufacturing | application(s) clinical research |
| solubility water: soluble (400 g plus 600 mL of water) | solubility methanol: soluble 26 mg/mL at 25 °C, ethylene glycol: soluble 79.1 mg/mL at 25 °C, water: soluble (678 g/l at 20 °C) | solubility H2O: 1 M, colorless, water: 678 g/L at 20 °C | solubility water: soluble (666 mg/ml, yielding a clear colorless solution) |
| pH 10.0-11.5 | pH 10.5-12 | pH 10.5-12.0 (1 M in H2O) | pH 10.0-11.5 |
Description générale
Application
- As a component of lysis buffer for cell disruption[4][5]
- In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)[6][5]
- In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC[7]
- In capillary electrochromatography and UV analysis of tocopherols and tocotrienols[8]
Conditionnement
Remarque sur l'analyse
Autres remarques
Pour les applications précises, utiliser un pH-mètre soigneusement calibré avec une électrode de verre au calomel.
Informations légales
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Gloves
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Should it be stored at 4℃??
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This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, the Safety Data Sheet (SDS).
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