There is no specific diluent recommended for this product. However, most researchers choose to dilute primary antibodies in PBS containing 1% BSA. BSA may be somewhat more stable than milk. There are older publications that suggest using 1% serum from the host of the selected secondary antibody in PBS.
SAB3500356
Anti-MDA5 antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-IFIH1, Anti-Interferon-induced with helicase C domain protein 1, Anti-Melanoma differentiation-associated protein 5
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| Size/SKU | Availability | Price |
|---|---|---|
100 μg | Check Cart for Availability | $584.00 |
About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA (i), IF, IHC, WB
Citations:
7
$584.00
Check Cart for Availability
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
predicted mol wt 113 kDa
species reactivity
mouse, human
technique(s)
immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... IFIH1(64135)
General description
Melanoma differentiation-associated protein 5 (MDA5), also known as interferon induced with helicase C domain 1 (IFIH1), is a cytosolic RNA sensor. It is encoded by the gene mapped to human chromosome 2q24.2. The encoded protein is a member of the retinoic acid-inducible gene I-like receptor family. MDA5 is characterized with both caspase recruitment domain and putative DExH group RNA helicase domains.
Immunogen
MDA5 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human MDA5.
Application
Anti-MDA5 antibody produced in rabbit has been used in western blot analysis.
Biochem/physiol Actions
Melanoma differentiation-associated protein 5 (MDA5) plays a vital role in antiviral innate immune responses. In addition, the encoded protein also activates interferon regulatory factors 3 and 7 and nuclear factor-κB (NF-κB) via a mitochondrial viral signaling protein, inducing antiviral and proapoptotic responses, which is associated with various autoimmune diseases, such as type 1 diabetes (T1D), Graves′ disease (GD), multiple sclerosis (MS), psoriasis and rheumatoid arthritis (RA). Polymorphism in the gene is associated with the development of generalized vitiligo (GV).
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Supplied in PBS with 0.02% sodium azide.
Other Notes
The action of this antibody can be blocked using blocking peptide SBP3500356.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description affinity isolated antibody, buffered aqueous solution | description affinity isolated antibody, buffered aqueous solution | description affinity isolated antibody, buffered aqueous solution | description affinity isolated antibody, buffered aqueous solution |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Do you recommend diluting the antibody (for a western blot) in 5% milk or BSA?
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