R2283
Pepsin Reagent, Ready to Use, Antigen Retriever
suitable for immunohistochemistry
Synonym(s):
pepsin
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15 ML
$99.90
100 ML
$211.00
About This Item
NACRES:
NA.47
UNSPSC Code:
12171500
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Product Name
Pepsin Reagent, Ready to Use, Antigen Retriever, suitable for immunohistochemistry
biological source
Porcine stomach
application(s)
hematology
histology
storage temp.
2-8°C
Quality Level
1 of 4
This Item | H3292 | CSPD-RO | GE27-9500-01 |
|---|---|---|---|
| biological source Porcine stomach | biological source - | biological source - | biological source - |
| application(s) hematology | application(s) hematology | application(s) - | application(s) - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level - |
![CSPD ready-to-use Disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo [3.3.1.13,7]decan}-4-yl)phenyl phosphate](/deepweb/assets/sigmaaldrich/product/images/352/091/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1/640/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1.jpg)
Application
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to the application of antibodies in immunohistochemistry (IHC).It has been used to enhance the permeabilization of fungal cell walls during the fluorescence in situ hybridization (FISH) process and utilized during the RNA scope in situ hybridization process to perform antigen retrieval.[1][2]
General description
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to the application of antibodies. In immunohistochemistry (IHC), the most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves the accessibility of antibodies to tissue antigens. Ready to use pepsin reagent, antigen retriever has been used for antigen unmasking in immunohistochemistry.[3][4][5]
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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